Cj0011c, a Periplasmic Single- and Double-Stranded DNA-Binding Protein, Contributes to Natural Transformation in Campylobacter jejuni

dc.contributor.author Jeon, Byeonghwa
dc.contributor.author Zhang, Qijing
dc.contributor.author Zhang, Qijing
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-18T23:27:08.000
dc.date.accessioned 2020-07-07T05:14:44Z
dc.date.available 2020-07-07T05:14:44Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2007
dc.date.issued 2007-10-01
dc.description.abstract <p><em>Campylobacter jejuni</em> is an important bacterial pathogen causing gastroenteritis in humans. <em>C. jejuni</em> is capable of natural transformation, which is considered a major mechanism mediating horizontal gene transfer and generating genetic diversity. Despite recent efforts to elucidate the transformation mechanisms of <em>C. jejuni</em>, the process of DNA binding and uptake in this organism is still not well understood. In this study, we report a previously unrecognized DNA-binding protein (Cj0011c) in <em>C. jejuni</em> that contributes to natural transformation. Cj0011c is a small protein (79 amino acids) with a partial sequence homology to the C-terminal region of ComEA in <em>Bacillus subtilis</em>. Cj0011c bound to both single- and double-stranded DNA. The DNA-binding activity of Cj0011c was demonstrated with a variety of DNAs prepared from <em>C. jejuni</em> or <em>Escherichia coli</em>, suggesting that the DNA binding of Cj0011c is not sequence dependent. Deletion of the cj0011c gene from <em>C. jejuni</em> resulted in 10- to 50-fold reductions in the natural transformation frequency. Different from the <em>B. subtilis</em> ComEA, which is an integral membrane protein, Cj0011c is localized in the periplasmic space of <em>C. jejuni</em>. These results indicate that Cj0011c functions as a periplasmic DNA receptor contributing to the natural transformation of <em>C. jejuni</em>.</p>
dc.description.comments <p>This article is published as Jeon, Byeonghwa, and Qijing Zhang. "Cj0011c, a periplasmic single-and double-stranded DNA-binding protein, contributes to natural transformation in Campylobacter jejuni." Journal of bacteriology 189, no. 20 (2007): 7399-7407.doi: <a href="http://dx.doi.org/10.1128/JB.01012-07" target="_blank" title=" ">10.1128/JB.01012-07</a>. Posted with permission.</p>
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dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/167/
dc.identifier.articleid 1167
dc.identifier.contextkey 10836897
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/167
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92273
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/167/2007_Zhang_PeriplasmicSingle.pdf|||Fri Jan 14 21:04:37 UTC 2022
dc.source.uri 10.1128/JB.01012-07
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Molecular Genetics
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Cj0011c, a Periplasmic Single- and Double-Stranded DNA-Binding Protein, Contributes to Natural Transformation in Campylobacter jejuni
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1c6a5dfc-c604-457f-85be-122910db782e
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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