Concerns about environmental pollution from nitrate contamination of groundwater, resulting from crop production presents an urgent need for reevaluation and management of the use of N fertilizers. Millions of tons of by-products generated yearly by biotechnology industries have potential for use as soil amendments, and with some treatments can be used as organic N fertilizers. Laboratory and greenhouse studies were conducted to formulate organic fertilizers with biotechnology by-products generated by industries in Iowa, and to assess their potentials as N sources for corn (Zea mays L.). More than 50 different mixtures were prepared using 18 by-products. Thirty of these mixtures were enriched with urea or urea ammonium nitrate. Five N-enriched mixtures made from five biotechnology by-products were selected for greenhouse experiment. About 1 kg of each was prepared. Selections were based on both physical and chemical properties, including odor. The pH of these mixtures were amended with Ca(OH)2 Mixtures were air dried at room temperature (22 [+or -] 2°C) then incubated under aerobic conditions for 14 d. The treatment was compared with the conventional N fertilizer, urea ammonium nitrate (UAN). Corn was grown on three Iowa Surface soils treated at six rates of N ranging from 0- 500 mg N pot−1 (1 kg soil) and harvested after 40 d. Results showed that both dry matter and N yield increased with increasing the rates of N application. Percentage of N recovered decreased with increasing N rates. There was no significant difference among the mixtures, and UAN. Soils affected the dry matter yield and yield of N but had no effect on percentage of N recovered. Soil pH was not significantly affected. Results indicated that formulation of N-enriched organic fertilizers with biotechnology by-products is feasible.