Analysis of ALS-associated genes in the zebrafish Danio rerio

dc.contributor.author Smith, Courtney
dc.contributor.department Genetics, Developmental and Cell Biology
dc.date 2018-02-18T16:49:27.000
dc.date.accessioned 2020-07-07T05:12:02Z
dc.date.available 2020-07-07T05:12:02Z
dc.date.issued 2017-04-11
dc.description.abstract <p>Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease characterized by the deterioration of the upper and lower motor neurons in the central nervous system. Although the pathogenesis of ALS is largely unknown, exome-sequencing studies have revealed new genes with mutations that are linked to familial or sporadic cases. The current challenge is to understand the in vivo function of these genes, with the hope that this insight will shed light on their connection to ALS pathology. The zebrafish is a great model organism to study this disease because its genome has been sequenced and is very similar to that of humans. To begin our investigation, we used in situ hybridization to visualize the expression of 21 genes throughout zebrafish development. Specifically, we focus on expression in the motor neurons, as these are the cells that die in ALS. Next, we have used clustered regulatory interspaced short palindromic repeats (CRISPRs) technology to engineer targeted mutations in a few of these ALS-linked genes in zebrafish. Ultimately, these experiments in zebrafish will provide insight into the role of these genes in motor neuron development and, we hope, ultimately point us toward a definitive link with ALS.</p>
dc.identifier archive/lib.dr.iastate.edu/undergradresearch_symposium/2017/presentations/83/
dc.identifier.articleid 1263
dc.identifier.contextkey 10453741
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath undergradresearch_symposium/2017/presentations/83
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91852
dc.relation.ispartofseries Symposium on Undergraduate Research and Creative Expression
dc.source.bitstream archive/lib.dr.iastate.edu/undergradresearch_symposium/2017/presentations/83/Session_20IV.C.1._20Smith.pptx|||Sat Jan 15 02:09:34 UTC 2022
dc.subject.disciplines Genetics
dc.title Analysis of ALS-associated genes in the zebrafish Danio rerio
dc.type event
dc.type.genre event
dspace.entity.type Publication
relation.isSeriesOfPublication 6730f354-97b8-4408-bad3-7e5c3b2fca9d
thesis.degree.discipline Genetics
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