Steady-State and Time-Resolved Spectroscopy of F420 Extracted from Methanogen Cells

Date
1999
Authors
Ashby, K.
Kraus, George
Kraus, George
Petrich, J.
Casey, T.
Rasmussen, M.
Carpenter, Susan
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Abstract

The methanogen electron carrier F420 was extracted from a methanogen cell culture, Methanobrevibacter ruminantium, and separated from other cell components by a single anion exchange chromatographic process. The extent of separation was determined spectroscopically. The fluorescence lifetime of F420 at pH 7.5 is ~4.2 nanoseconds.

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