Mast cell density and substance P-like immunoreactivity during the initiation and progression of lung lesions in ovineMannheimia (Pasteurella) haemolytica pneumonia

dc.contributor.author Gallup, Jack
dc.contributor.author Ramírez-Romero, Rafael
dc.contributor.author Brogden, Kim
dc.contributor.author Gallup, Jack
dc.contributor.author Ackermann, Mark
dc.contributor.author Sonea, Ioana
dc.contributor.author Ackermann, Mark
dc.contributor.department Veterinary Pathology
dc.date 2018-02-13T06:26:54.000
dc.date.accessioned 2020-07-07T05:15:53Z
dc.date.available 2020-07-07T05:15:53Z
dc.date.embargo 2013-02-26
dc.date.issued 2001-06-01
dc.description.abstract <p>To determine the density of mast cells (MCs) and the extent of substance P (SP) immunoreactivity during initiation and progression of pneumonic pasteurellosis (PP), 18 lambs were inoculated intrabronchially with<em>Mannheimia</em> (<em>Pasteurella</em>) <em>haemolytica</em> or saline, and lung tissue was collected at 1, 15 and 45 days post-inoculation (n=3, each group). Additionally, the left (non-inoculated) contralateral lungs in bacteria-inoculated animals were collected as controls. At 1 day after bacterial inoculation the lungs had typical <em>M. haemolytica</em>lesions. These pneumonic lesions had fewer numbers of MCs and reduced histamine content. Macrophages infiltrating some of the inflamed areas were strongly immunoreactive for SP. At 15 days, MCs remained scarce at sites where lung damage persisted, i.e. pyogranulomatous foci, but were increased in number in areas of interstitial damage. Pulmonary ganglion neurons were strongly immunoreactive for SP. By 45 days the fibrosing changes became more defined as pleural fibrosis, fibrosing alveolitis, alveolar epithelial hyperplasia and bronchiolitis obliterans. These lungs had increased numbers of MCs, but histamine content was not different from saline- and non-inoculated left lungs. Substance P immunoreactivity occurred only in nerves and was scarce and mild. This work demonstrates that MC density decreases initially with PP, but increases with progression of PP. SP fibres tend to be decreased during the initiation and at 45 days of PP, but other cells, such as macrophages and neuronal ganglion cells, produce substance P during progression of PP and thereby constitute an additional source of substance P.</p>
dc.description.comments <p>This article is from <em>Microbial Pathogenesis </em>30, no. 6 (June 2001): 325–335, doi:<a href="http://dx.doi.org/10.1006/mpat.2000.0437" id="x-ddDoi" target="_blank">10.1006/mpat.2000.0437</a>.</p>
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dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/39/
dc.identifier.articleid 1043
dc.identifier.contextkey 3792462
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/39
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92463
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/39/2001_RamirezRomero_MastCellDensity.pdf|||Fri Jan 14 23:54:19 UTC 2022
dc.source.uri 10.1006/mpat.2000.0437
dc.subject.disciplines Medical Sciences
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords Mannheimia (Pasteurella) haemolytica
dc.subject.keywords pneumonic pasteurellosis
dc.subject.keywords pathogenesis
dc.subject.keywords mast cells
dc.subject.keywords substance P
dc.subject.keywords Biomedical Sciences
dc.title Mast cell density and substance P-like immunoreactivity during the initiation and progression of lung lesions in ovineMannheimia (Pasteurella) haemolytica pneumonia
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isAuthorOfPublication 86c1ed73-b60d-48ce-8f35-449bc320a693
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
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