In Situ Temporospatial Characterization of the Glial Response to Prion Infection

dc.contributor.author Michael, Alyona
dc.contributor.author Greenlee, Justin
dc.contributor.author Harm, Tyler
dc.contributor.author Moore, S. Jo
dc.contributor.author Smith, Jodi
dc.contributor.author Zhang, Min
dc.contributor.author Lind, Melissa
dc.contributor.author Greenlee, M. Heather
dc.contributor.author Smith, Jodi
dc.contributor.department Biomedical Sciences
dc.contributor.department Veterinary Pathology
dc.contributor.department Statistics
dc.date 2021-08-03T21:58:46.000
dc.date.accessioned 2021-08-15T02:09:59Z
dc.date.available 2021-08-15T02:09:59Z
dc.date.issued 2020-01-01
dc.description.abstract <p>Mammalian transmissible spongiform encephalopathies (TSEs) display marked activation of astrocytes and microglia that precedes neuronal loss. Investigation of clinical parallels between TSEs and other neurodegenerative protein misfolding diseases, such as Alzheimer’s disease, has revealed similar patterns of neuroinflammatory responses to the accumulation of self-propagating amyloids. The contribution of glial activation to the progression of protein misfolding diseases is incompletely understood, with evidence for mediation of both protective and deleterious effects. Glial populations are heterogeneously distributed throughout the brain and capable of dynamic transitions along a spectrum of functional activation states between pro- and antiinflammatory polarization extremes. Using a murine model of Rocky Mountain Laboratory scrapie, the neuroinflammatory response to prion infection was characterized by evaluating glial activation across 15 brain regions over time and correlating it to traditional markers of prion neuropathology, including vacuolation and PrP<sup>Sc</sup> deposition. Quantitative immunohistochemistry was used to evaluate glial expression of iNOS and Arg1, markers of classical and alternative glial activation, respectively. The results indicate progressive upregulation of iNOS in microglia and a mixed astrocytic profile featuring iNOS expression in white matter tracts and detection of Arg1-positive populations throughout the brain. These data establish a temporospatial lesion profile for this prion infection model and demonstrate evidence of multiple glial activation states.</p>
dc.description.comments <p>This article is published as Michael, Alyona V., Justin J. Greenlee, Tyler A. Harm, S. Jo Moore, Min Zhang, Melissa S. Lind, M. Heather West Greenlee, and Jodi D. Smith. "In Situ Temporospatial Characterization of the Glial Response to Prion Infection." <em>Veterinary Pathology</em> 57, no. 1 (2020): 90-107. DOI: <a href="https://doi.org/10.1177%2F0300985819861708" target="_blank">10.1177%2F0300985819861708</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/121/
dc.identifier.articleid 1120
dc.identifier.contextkey 24165667
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/121
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/kv7k9A4v
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/121/2020_SmithJodi_InSitu.pdf|||Fri Jan 14 19:13:05 UTC 2022
dc.source.uri 10.1177%2F0300985819861708
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords arg1
dc.subject.keywords astrocytes
dc.subject.keywords iNOS
dc.subject.keywords immunohistochemistry
dc.subject.keywords microglia
dc.subject.keywords mouse
dc.subject.keywords prion
dc.subject.keywords scrapie
dc.title In Situ Temporospatial Characterization of the Glial Response to Prion Infection
dc.type article
dc.type.genre article
dspace.entity.type Publication
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