Diversity and Relationships among U.S. Maize Inbreds Revealed by Restriction Fragment Length Polymorphisms

dc.contributor.author Melchinger, A. E.
dc.contributor.author Messmer, M. M.
dc.contributor.author Lamkey, Kendall
dc.contributor.author Lee, M.
dc.contributor.author Woodman, W. L.
dc.contributor.author Lamkey, K. R.
dc.contributor.department Agronomy
dc.date 2018-02-19T00:17:18.000
dc.date.accessioned 2020-06-29T23:03:43Z
dc.date.available 2020-06-29T23:03:43Z
dc.date.issued 1991
dc.description.abstract <p>Restriction fragment length polymorphisms (RFLPs) have been proposed as molecular markers for characterizing the genetic diversity in maize (<em>Zea mays</em> L.). The objectives of this study were to evaluate the usefulness of RFLP data for (i) elucidating heterotic patterns among maize inbreds and (ii) assessing genetic similarity among related and unrelated lines. Thirty-two maize inbreds from the U.S. Corn Belt were analyzed for RFLPs with two restriction enzymes and 83 DNA probes distributed over the maize genome. Eighty-two probes detected polymorphisms with at least one enzyme. On average, 4.3 variants were found per probe-enzyme combination across all 32 inbreds. Genetic distances among lines, estimated from RFLP data as Rogers' distance (RD), revealed considerable diversity among lines from Iowa Stiff Stalk Synthetic (BSSS), Reid Yellow Dent (RYD), and Lancaster Sure Crop (LSC). Lines from different heterotic groups had a slightly greater RD mean than unrelated lines from the same heterotic group, yet differences were small when compared with the wide range of RDs for individual lines combinations within each group. RDs between related lines agreed well with expectations based on coancestry coefficients determined from pedigree data with few exceptions. Principal component analyses of RFLP data resulted in a separate grouping of lines from BSSS/RYD and LSC. Dispersion of lines of miscellaneous origins was generally consistent with expectations based on known breeding behavior and pedigrees. Results from this study suggest that RFLP data can be used for assigning inbreds into heterotic groups and quantifying genetic similarity between related lines, but it seems that a large number of probe-enzyme combinations are required to obtain reliable estimates of genetic distance.</p>
dc.description.comments <p>This article is published as Melchinger, A. E., M. M. Messmer, M. Lee, W. L. Woodman, and K. R. Lamkey. "Diversity and relationships among US maize inbreds revealed by restriction fragment length polymorphisms." Crop Science 31, no. 3 (1991): 669-678. doi: <a href="http://dx.doi.org/10.2135/cropsci1991.0011183X003100030025x" target="_blank" title="Diversity and Relationships among U.S. Maize Inbreds Revealed by Restriction Fragment Length Polymorphisms">10.2135/cropsci1991.0011183X003100030025x</a>. Posted with permission.</p>
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dc.identifier archive/lib.dr.iastate.edu/agron_pubs/248/
dc.identifier.articleid 1251
dc.identifier.contextkey 10945029
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/248
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/4586
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/248/1991_Lamkey_DiversityRelationships.pdf|||Fri Jan 14 22:54:49 UTC 2022
dc.source.uri 10.2135/cropsci1991.0011183X003100030025x
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Molecular Genetics
dc.subject.disciplines Plant Breeding and Genetics
dc.title Diversity and Relationships among U.S. Maize Inbreds Revealed by Restriction Fragment Length Polymorphisms
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication fadecd27-89f7-4164-8981-ce39d1a066e1
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
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