A microflow cytometer for optical analysis of phytoplankton

dc.contributor.author Golden, Joel
dc.contributor.author Hashemi, Nicole
dc.contributor.author Hashemi, Nicole
dc.contributor.author Erickson, Jeffrey
dc.contributor.author Ligler, Frances
dc.contributor.department Mechanical Engineering
dc.date 2019-07-18T06:31:37.000
dc.date.accessioned 2020-06-30T06:01:52Z
dc.date.available 2020-06-30T06:01:52Z
dc.date.embargo 2015-03-24
dc.date.issued 2012-02-01
dc.description.abstract <p>Analysis of the intrinsic scatter and fluorescence profiles of marine algae can be used for general classification of organisms based on cell size and fluorescence properties. We describe the design and fabrication of a Microflow Cytometer on a chip for characterization of phytoplankton. The Microflow Cytometer measured distinct side-scatter and fluorescence properties of Synechococcus sp., Nitzschia d., and Thalassiosira p. Measurements were confirmed using the benchtop Accuri C6 flow cytometer. The Microflow Cytometer proved sensitive enough to detect and characterize picoplankton with diameter approximately 1 mm and larger phytoplankton of up to 80 mm in length. The wide range in size discrimination coupled with detection of intrinsic fluorescent pigments suggests that this Microflow Cytometer will be able to distinguish different populations of phytoplankton on unmanned underwater vehicles. Reversing the orientation of the grooves in the channel walls returns the sample stream to its original unsheathed position allowing separation of the sample stream from the sheath streams and the recycling of the sheath fluid.</p>
dc.description.comments <p>This is a conference proceeding from <em>Proceeding of SPIE</em> 8212 (2012): 1, <a href="http://dx.doi.org/10.1117/12.905679" target="_blank">doi:10.1117/12.905679</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/me_conf/156/
dc.identifier.articleid 1148
dc.identifier.contextkey 6890876
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath me_conf/156
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/54801
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/me_conf/156/2012_Hashemi_MicroflowCytometer.pdf|||Fri Jan 14 20:43:34 UTC 2022
dc.source.uri 10.1117/12.905679
dc.subject.disciplines Acoustics, Dynamics, and Controls
dc.subject.disciplines Marine Biology
dc.subject.disciplines Other Oceanography and Atmospheric Sciences and Meteorology
dc.subject.keywords flow cytometry
dc.subject.keywords microfluidics
dc.subject.keywords multi-analyte assay
dc.subject.keywords phytoplankton
dc.subject.keywords algae
dc.subject.keywords microflow cytometer
dc.title A microflow cytometer for optical analysis of phytoplankton
dc.type article
dc.type.genre conference
dspace.entity.type Publication
relation.isAuthorOfPublication f1ba9f2a-a64d-43aa-97ca-0d72675c4f2e
relation.isOrgUnitOfPublication 6d38ab0f-8cc2-4ad3-90b1-67a60c5a6f59
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