Sampling guidelines for oral fluid-based surveys of group-housed animals Rotolo, Marisa Sun, Yaxuan Wang, Chong Wang, Chong Gimenez-Lirola, Luis Baum, David Gauger, Phillip Harmon, Karen Hoogland, Marlin Main, Rodger Zimmerman, Jeffrey
dc.contributor.department Statistics
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine 2018-06-18T20:34:05.000 2020-07-07T05:12:39Z 2020-07-07T05:12:39Z Sun Jan 01 00:00:00 UTC 2017 2017-09-01
dc.description.abstract <p>Formulas and software for calculating sample size for surveys based on individual animal samples are readily available. However, sample size formulas are not available for oral fluids and other aggregate samples that are increasingly used in production settings. Therefore, the objective of this study was to develop sampling guidelines for oral fluid-based porcine reproductive and respiratory syndrome virus (PRRSV) surveys in commercial swine farms. Oral fluid samples were collected in 9 weekly samplings from all pens in 3 barns on one production site beginning shortly after placement of weaned pigs. Samples (n = 972) were tested by real-time reverse-transcription PCR (RT-rtPCR) and the binary results analyzed using a piecewise exponential survival model for interval-censored, time-to-event data with misclassification. Thereafter, simulation studies were used to study the barn-level probability of PRRSV detection as a function of sample size, sample allocation (simple random sampling vs fixed spatial sampling), assay diagnostic sensitivity and specificity, and pen-level prevalence. These studies provided estimates of the probability of detection by sample size and within-barn prevalence. Detection using fixed spatial sampling was as good as, or better than, simple random sampling. Sampling multiple barns on a site increased the probability of detection with the number of barns sampled. These results are relevant to PRRSV control or elimination projects at the herd, regional, or national levels, but the results are also broadly applicable to contagious pathogens of swine for which oral fluid tests of equivalent performance are available.</p>
dc.description.comments <p>This article is published as Rotolo, Marisa L., Yaxuan Sun, Chong Wang, Luis Giménez-Lirola, David H. Baum, Phillip C. Gauger, Karen M. Harmon, Marlin Hoogland, Rodger Main, and Jeffrey J. Zimmerman. "Sampling guidelines for oral fluid-based surveys of group-housed animals." <em>Veterinary microbiology</em> 209 (2017): 20-29. doi: <a href="" target="_blank" title="Persistent link using digital object identifier">10.1016/j.vetmic.2017.02.004</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 1112
dc.identifier.contextkey 12332089
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/113
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 18:47:15 UTC 2022
dc.source.uri 10.1016/j.vetmic.2017.02.004
dc.subject.disciplines Design of Experiments and Sample Surveys
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Statistical Models
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords Oral fluid
dc.subject.keywords Sampling
dc.subject.keywords Surveillance
dc.subject.keywords Sample size
dc.subject.keywords PRRSV
dc.subject.keywords Modeling
dc.subject.keywords Probability of detection
dc.subject.keywords Monitoring
dc.title Sampling guidelines for oral fluid-based surveys of group-housed animals
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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