Does Circulating Antibody Play a Role in the Protection of Piglets against Porcine Epidemic Diarrhea Virus?

dc.contributor.author Poonsuk, Korakrit
dc.contributor.author Gimenez-Lirola, Luis Gabriel
dc.contributor.author Zhang, Jianqiang
dc.contributor.author Wang, Chong
dc.contributor.author Arruda, Paulo
dc.contributor.author Chen, Qi
dc.contributor.author da Silva Carrion, Lucas Correa
dc.contributor.author Magtoto, Ronaldo
dc.contributor.author Pineyro, Pablo
dc.contributor.author Sarmento, Luciana
dc.contributor.author Wang, Chong
dc.contributor.author Sun, Yaxuan
dc.contributor.author Madson, Darin
dc.contributor.author Johnson, John
dc.contributor.author Yoon, Kyoung-Jin
dc.contributor.author Zimmerman, Jeffrey
dc.contributor.author Main, Rodger
dc.contributor.department Statistics
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2018-06-18T20:32:01.000
dc.date.accessioned 2020-07-07T05:12:38Z
dc.date.available 2020-07-07T05:12:38Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-04-06
dc.description.abstract <p>The contribution of circulating antibody to the protection of naïve piglets against porcine epidemic diarrhea virus (PEDV) was evaluated using a passive antibody transfer model. Piglets (n = 62) derived from 6 sows were assigned to one of 6 different treatments using a randomized block design which provided for allocation of all treatments to all sows' litters. Each treatment was designed to achieve a different level of circulating anti-PEDV antibody via intraperitoneally administration of concentrated serum antibody. Piglets were orally inoculated with PEDV (USA/IN/2013/19338E, 1 x 103 TCID50 per piglet) 24 hours later and then monitored for 14 days. Piglets remained with their dam throughout the experiment. Sow milk samples, piglet fecal samples, and data on piglet clinical signs, body weight, and body temperature were collected daily. Fecal samples were tested by PEDV real-time reverse transcriptase PCR. Serum, colostrum, and milk were tested for PEDV IgG, IgA, and virus-neutralizing antibody. The data were evaluated for the effects of systemic PEDV antibody levels on growth, body temperature, fecal shedding, survival, and antibody response. The analysis showed that circulating antibody partially ameliorated the effect of PEDV infection. Specifically, antibody-positive groups returned to normal body temperature faster and demonstrated a higher rate of survivability than piglets without PEDV antibody. When combined with previous literature on PEDV, it can be concluded that both systemic antibodies and maternal secretory IgA in milk contribute to the protection of the neonatal pig against PEDV infections. Overall, the results of this experiment suggested that passively administered circulating antibodies contributed to the protection of neonatal piglets against PEDV infection.</p>
dc.description.comments <p>This article is published as Poonsuk, Korakrit, Luis Gabriel Giménez-Lirola, Jianqiang Zhang, Paolo Arruda, Qi Chen, Lucas Correa da Silva Carrion, Ronaldo Magtoto et al. "Does circulating antibody play a role in the protection of piglets against porcine epidemic diarrhea virus?." <em>PloS one</em> 11, no. 4 (2016): e0153041. doi: <a href="https://doi.org/10.1371/journal.pone.0153041">10.1371/journal.pone.0153041</a>.</p>
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dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/109/
dc.identifier.articleid 1116
dc.identifier.contextkey 12334105
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/109
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91950
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/109/2016_Wang_DoesCirculating.PDF|||Fri Jan 14 18:30:39 UTC 2022
dc.source.uri 10.1371/journal.pone.0153041
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Statistical Models
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Does Circulating Antibody Play a Role in the Protection of Piglets against Porcine Epidemic Diarrhea Virus?
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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