Reconstruction of ribosomal subunits and rDNA chromatin imaged by scanning force microscopy

Date
1996
Authors
Dobbs, Drena
Fritzsche, Wolfgang
Martin, Linda
Dobbs, Drena
Jondle, Daniel
Miller, Richard
Henderson, Eric
Vesenka, James
Henderson, Eric
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Zoology and Genetics
Organizational Unit
Mathematics
Organizational Unit
Journal Issue
Series
Department
Zoology and GeneticsMathematics
Abstract

Scanning force microscopy (SFM) reveals surface topography by scanning a sharp tip in close proximity to the sample. Due to tip–sample interaction, artificial broadening of the real surface structure with the tip geometry occurs. One approach for image reconstruction is the use of calibration standards, preferably in the size range of the samples. In the present study, an image reconstruction method based on colloidal gold as a geometric standard was used to reconstruct SFM images of biomolecules. Sample and calibration standard size were in the nanometer range, and the standards were coadsorbed with the specimen. Raw and reconstructed images of the biomolecules were compared, and the reconstruction was characterized by difference images as well as determination of the difference volume. The application of image reconstruction based on colloidal gold as a calibration standard for SFM of biomolecules is discussed.

Comments

This article is from Journal of Vacuum Science & Technology B 14 (1996): 1405, doi: 10.1116/1.589108. Posted with permission.

Description
Keywords
Citation
DOI
Collections