Reconstruction of ribosomal subunits and rDNA chromatin imaged by scanning force microscopy

dc.contributor.author Dobbs, Drena
dc.contributor.author Fritzsche, Wolfgang
dc.contributor.author Martin, Linda
dc.contributor.author Dobbs, Drena
dc.contributor.author Jondle, Daniel
dc.contributor.author Miller, Richard
dc.contributor.author Henderson, Eric
dc.contributor.author Vesenka, James
dc.contributor.author Henderson, Eric
dc.contributor.department Zoology and Genetics
dc.contributor.department Mathematics
dc.date 2018-02-18T05:05:26.000
dc.date.accessioned 2020-07-07T05:16:47Z
dc.date.available 2020-07-07T05:16:47Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 1996
dc.date.issued 1996
dc.description.abstract <p>Scanning force microscopy (SFM) reveals surface topography by scanning a sharp tip in close proximity to the sample. Due to tip–sample interaction, artificial broadening of the real surface structure with the tip geometry occurs. One approach for image reconstruction is the use of calibration standards, preferably in the size range of the samples. In the present study, an image reconstruction method based on colloidal gold as a geometric standard was used to reconstruct SFM images of biomolecules. Sample and calibration standard size were in the nanometer range, and the standards were coadsorbed with the specimen. Raw and reconstructed images of the biomolecules were compared, and the reconstruction was characterized by difference images as well as determination of the difference volume. The application of image reconstruction based on colloidal gold as a calibration standard for SFM of biomolecules is discussed.</p>
dc.description.comments <p>This article is from <em>Journal of Vacuum Science & Technology B </em>14 (1996): 1405, doi: <a href="http://dx.doi.org/10.1116/1.589108">10.1116/1.589108</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/zool_pubs/16/
dc.identifier.articleid 1008
dc.identifier.contextkey 9760536
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath zool_pubs/16
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92624
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/zool_pubs/16/1996_Dobbs_ReconstructionRibosomal.pdf|||Fri Jan 14 20:52:13 UTC 2022
dc.source.uri 10.1116/1.589108
dc.subject.disciplines Cell Biology
dc.subject.disciplines Developmental Biology
dc.subject.disciplines Geometry and Topology
dc.subject.disciplines Molecular Genetics
dc.title Reconstruction of ribosomal subunits and rDNA chromatin imaged by scanning force microscopy
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 7e096c4f-9007-41e4-9414-989c3ea9bc88
relation.isAuthorOfPublication 68bc511a-afd6-4353-ae20-066de33682b9
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relation.isOrgUnitOfPublication 82295b2b-0f85-4929-9659-075c93e82c48
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