Diagnostic evaluation of assays for detection of antibodies against porcine epidemic diarrhea virus (PEDV) in pigs exposed to different PEDV strains

dc.contributor.author Gerber, Priscilla
dc.contributor.author Lelli, Davide
dc.contributor.author Wang, Chong
dc.contributor.author Zhang, Jianqiang
dc.contributor.author Strandbygaard, Bertel
dc.contributor.author Moreno, Ana
dc.contributor.author Lavazza, Antonio
dc.contributor.author Perulli, Simona
dc.contributor.author Bøtner, Anette
dc.contributor.author Comtet, Loic
dc.contributor.author Roche, Mickael
dc.contributor.author Pourquier, Philippe
dc.contributor.author Wang, Chong
dc.contributor.author Opriessnig, Tanja
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2018-06-18T20:31:32.000
dc.date.accessioned 2020-07-07T05:12:37Z
dc.date.available 2020-07-07T05:12:37Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-12-01
dc.description.abstract <p>Porcine epidemic diarrhea virus (PEDV) has caused economic losses in the Americas, Asia and Europe in recent years. Reliable serological assays are essential for epidemiological studies and vaccine evaluation. The objective of this study was to compare the ability of five enzymelinked immunosorbent assays (ELISAs) to detect antibodies against different PEDV strains in pig serum. A total of 732 serum samples from North American or European pigs were tested. Samples included experimental samples from pigs infected with classical (G1a PEDV) or variant genogroup 1 PEDV (G1b PEDV), pandemic genogroup 2 PEDV (G2b PEDV) or noninfected controls. Field samples from herds with confirmed or unknown PEDV exposure were also used. Three indirect ELISAs based on G2b antigens (ELISAs 1, 2 and 3), a competitive ELISA based on the G2b antigen (ELISA 4) and a competitive ELISA based on the G1a antigen (ELISA 5) were compared. Overall, the tests had a moderate agreement (κ = 0.61). G1a PEDV infected pigs were earliest detected by ELISA 3, G1b PEDV infected pigs were earliest detected by ELISAs 4 and 5 and the performance of all tests was similar for the G2b PEDV group. ELISA 1 showed the overall lowest detection on experimentally and field derived samples. Diagnostic sensitivity and specificity with a 95% probability interval were estimated to be 68.2% (62.1 – 74.4%) and 97.5% (95.2 – 99.0%) for ELISA 1, 73.7% (71.5 – 79.6%) and 98.4% (96.6 – 99.5%) for ELISA 2, 86.2% (81.1 – 90.6%) and 91.6% (87.7 – 94.8%) for ELISA 3, 78.3% (72.8 – 83.5%) and 99.7% (98.2 – 100%) for ELISA 4, and 93.5% (90.3 – 96.0%) and 91.2% (83.8 – 97.9%) for ELISA 5. Differences in detection among assays seem to be more related to intrinsic factors of an assay than to the PEDV antigen used.</p>
dc.description.comments <p>This is a manuscript of an article published as Gerber, Priscilla F., Davide Lelli, Jianqiang Zhang, Bertel Strandbygaard, Ana Moreno, Antonio Lavazza, Simona Perulli et al. "Diagnostic evaluation of assays for detection of antibodies against porcine epidemic diarrhea virus (PEDV) in pigs exposed to different PEDV strains." <em>Preventive veterinary medicine</em> 135 (2016): 87-94. doi: <a href="https://doi.org/10.1016/j.prevetmed.2016.11.005" target="_blank" title="Persistent link using digital object identifier">10.1016/j.prevetmed.2016.11.005</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/108/
dc.identifier.articleid 1117
dc.identifier.contextkey 12334366
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/108
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91949
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/108/2016_Wang_DaignosticEvaluationManuscript.pdf|||Fri Jan 14 18:28:33 UTC 2022
dc.source.uri 10.1016/j.prevetmed.2016.11.005
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Statistics and Probability
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords Porcine epidemic diarrhea virus
dc.subject.keywords ELISA
dc.subject.keywords global comparison
dc.subject.keywords diagnosis
dc.title Diagnostic evaluation of assays for detection of antibodies against porcine epidemic diarrhea virus (PEDV) in pigs exposed to different PEDV strains
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication b715071c-c3bd-419c-b021-0ac4702f346a
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
File
Original bundle
Now showing 1 - 1 of 1
Name:
2016_Wang_DaignosticEvaluationManuscript.pdf
Size:
444.34 KB
Format:
Adobe Portable Document Format
Description:
Collections