An IoT-enabled paper sensor platform for real-time analysis of isothermal nucleic acid amplification tests

Date
2020-01-01
Authors
Liu, Mingdian
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Abstract

This work proposes a paper-based sensor system for point-of-care nucleic acid amplification tests. The system integrates a paper sensor chip and a compact fluorescence detector, which is built towards the Internet of Things (IoT) platform via an Arduino controller. The paper-based sensor provides the functions of reagent storage, sample transportation, and DNA amplification. The compact and low-cost fluorescence detector is capable of quantifying the amplicons of the loop-mediated isothermal amplification (LAMP) reaction in real-time. The results show that the paper-based sensor can detect multiple genes of the genomic DNA extracted from Escherichia coli and Campylobacter jejuni, with the concentration as low as 2 × 103 copies/μL. As a diagnosis tool, the sensor has the potential to measure multiple target genes simultaneously.

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Keywords
Loop-mediated isothermal amplification, Paper-based biosensor, Point-of-care diagnosis
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