Gene Expression Programs during Shoot, Root, and Callus Development in Arabidopsis Tissue Culture

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dc.contributor.author Che, Ping
dc.contributor.author Lall, Sonia
dc.contributor.author Nettleton, Dan
dc.contributor.author Nettleton, Dan
dc.contributor.author Howell, Stephen
dc.contributor.department Statistics
dc.contributor.department Plant Sciences Institute
dc.date 2019-09-13T05:31:36.000
dc.date.accessioned 2020-07-02T06:57:27Z
dc.date.available 2020-07-02T06:57:27Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2006
dc.date.issued 2006-06-01
dc.description.abstract <p>Shoots can be regenerated from Arabidopsis (<em>Arabidopsis</em> <em>thaliana</em>) root explants in tissue culture through a two-step process requiring preincubation on an auxin-rich callus induction medium. Regenerating tissues can be directed along different developmental pathways leading to the formation of shoots, new roots, or callus by transferring to the appropriate organ induction medium. Using gene-profiling methods, we identified groups of genes that serve as molecular signatures of the different developmental processes, i.e. genes that were specifically up- or down-regulated on one developmental pathway, but not on others. One transcription factor gene that was up-regulated during early shoot development was <em>RAP2.6L</em> (At5g13330), a member of the ERF (ethylene response factor) subfamily B-4 of the ERF/<em>APETALA2</em> transcription factor gene family. <em>RAP2.6L</em>functions in shoot regeneration because T-DNA knockdown mutations in the gene reduced the efficiency of shoot formation in tissue culture, but not normal embryo or seedling development. <em>RAP2.6L</em> promoter:<em>β</em>-glucuronidase fusions demonstrated that the up-regulation of the gene during shoot regeneration was, at least in part, transcriptionally controlled. The promoter:<em>β</em>-glucuronidase fusions also demonstrated that <em>RAP2.6L</em> expression was localized to the shoot and emerging leaves, but expression declined in the leaf lamina as leaves expanded. T-DNA knockdown mutations in <em>RAP2.6L</em> reduced the expression of many genes that are normally up-regulated during shoot development including <em>CUP-SHAPED COTYLEDON2</em> that is involved in shoot meristem specification. Thus, <em>RAP2.6L</em> appears to be part of a network involved in regulating the expression of many other genes in shoot regeneration.</p>
dc.description.comments This article is published as Che, Ping, Sonia Lall, Dan Nettleton, and Stephen H. Howell. "Gene expression programs during shoot, root, and callus development in Arabidopsis tissue culture." <em>Plant Physiology </em>141, no. 2 (2006): 620-637. doi: <a href="https://doi.org/10.1104/pp.106.081240" target="_blank">10.1104/pp.106.081240</a>. Posted with permission.
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/stat_las_pubs/251/
dc.identifier.articleid 1251
dc.identifier.contextkey 14925894
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath stat_las_pubs/251
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/90567
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/stat_las_pubs/251/2006_Nettleton_GeneExpression.pdf|||Fri Jan 14 22:57:59 UTC 2022
dc.source.uri 10.1104/pp.106.081240
dc.subject.disciplines Molecular Genetics
dc.subject.disciplines Plant Biology
dc.subject.disciplines Plant Breeding and Genetics
dc.title Gene Expression Programs during Shoot, Root, and Callus Development in Arabidopsis Tissue Culture
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 7d86677d-f28f-4ab1-8cf7-70378992f75b
relation.isOrgUnitOfPublication 264904d9-9e66-4169-8e11-034e537ddbca
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