Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry

dc.contributor.author Johnson, Timothy
dc.contributor.author Nolan, Lisa
dc.contributor.author Danzeisen, Jessica
dc.contributor.author Trampel, Darrell
dc.contributor.author Nolan, Lisa
dc.contributor.author Seemann, Torsten
dc.contributor.author Bager, Ragnhild
dc.contributor.author Bojesen, Anders
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-13T10:26:31.000
dc.date.accessioned 2020-07-07T05:14:57Z
dc.date.available 2020-07-07T05:14:57Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.embargo 2013-05-03
dc.date.issued 2013-01-24
dc.description.abstract <p>Peritonitis is the major disease problem of laying hens in commercial table egg and parent stock operations. Despite its importance, the etiology and pathogenesis of this disease have not been completely clarified. Although avian pathogenic <em>Escherichia coli</em> (APEC) isolates have been incriminated as the causative agent of laying hen peritonitis, <em>Gallibacterium anatis</em> are frequently isolated from peritonitis lesions. Despite recent studies suggesting a role for <em>G. anatis</em> in the pathogenesis of peritonitis, little is known about the organism’s virulence mechanisms, genomic composition and population dynamics. Here, we compared the genome sequences of three <em>G. anatis</em> isolates in an effort to understand its virulence mechanisms and identify novel antigenic traits. A multilocus sequence typing method was also established for <em>G. anatis</em> and used to characterize the genotypic relatedness of 71 isolates from commercial laying hens in Iowa and 18 international reference isolates. Genomic comparisons suggest that<em>G. anatis</em> is a highly diverse bacterial species, with some strains possessing previously described and potential virulence factors, but with a core genome containing several antigenic candidates. Multilocus sequence typing effectively distinguished 82 sequence types and several clonal complexes of <em>G. anatis</em>, and some clones seemed to predominate among <em>G. anatis</em> populations from commercial layers in Iowa. Biofilm formation and resistance to antimicrobial agents was also observed in several clades. Overall, the genomic diversity of <em>G. anatis</em> suggests that multiple lineages exist with differing pathogenic potential towards birds.</p>
dc.description.comments <p>This article is from <em>PLoS ONE </em>8, no. 1 (2013): e54844, doi:<a href="http://dx.doi.org/10.1371/journal.pone.0054844" target="_blank">10.1371/journal.pone.0054844</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/2/
dc.identifier.articleid 1002
dc.identifier.contextkey 4106654
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/2
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92309
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/2/2013_JohnsonTJ_GenomeAnalysisPhylogenetic.pdf|||Fri Jan 14 22:02:02 UTC 2022
dc.source.uri 10.1371/journal.pone.0054844
dc.subject.disciplines Genomics
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Genome Analysis and Phylogenetic Relatedness of Gallibacterium anatis Strains from Poultry
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 9e7506b4-e945-47cf-9195-e814dac6c9fd
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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