Application of PCR Assay to Differentiate Two Subtypes of Swine Influenza Viruses

dc.contributor.author Harmon, Karen
dc.contributor.author Yoon, Kyoung-Jin
dc.date 2018-02-13T00:12:58.000
dc.date.accessioned 2020-07-02T07:00:55Z
dc.date.available 2020-07-02T07:00:55Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2000
dc.date.embargo 2012-08-17
dc.date.issued 2000-01-01
dc.description.abstract <p>A reverse transcription-polymerase chain reaction (RTPCR) test was developed to differentiate between H1 and H3 subtypes of swine influenza virus (SIV). The sensitivity and specificity of this test was evaluated by comparing the results of the PCR test with subtyping results of an immunological assay performed at the National Veterinary Services Laboratories (NVSL). The test was performed on 68 eggderived SIV isolates, as well as directly on 30 lung homogenates. The data suggest that a PCR-based assay may be a reliable screening test for SIV both from egg fluid and directly from lung tissue.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/swinereports_1999/42/
dc.identifier.articleid 1038
dc.identifier.contextkey 3229637
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath swinereports_1999/42
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91210
dc.language.iso en
dc.relation.ispartofseries Animal Science Research Reports
dc.relation.ispartofseries ASL R1696
dc.source.bitstream archive/lib.dr.iastate.edu/swinereports_1999/42/asl_1696.pdf|||Sat Jan 15 00:11:49 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.keywords ASL R1696
dc.title Application of PCR Assay to Differentiate Two Subtypes of Swine Influenza Viruses
dc.type article
dc.type.genre health
dspace.entity.type Publication
relation.isSeriesOfPublication 7f3839b7-b833-4418-a6fa-adda2b23950a
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