Development of New Placental and Fetal Expressed Sequence Tags (EST) for Gene Discovery in Pig Reproduction Tuggle, C. Malchenko, S. Woods, R. Whitworth, K. Green, J. Prather, R. Fitzsimmons, C. Roberts, C. Casavant, T. Soares, M. 2018-02-12T23:58:52.000 2020-07-02T07:01:03Z 2020-07-02T07:01:03Z Mon Jan 01 00:00:00 UTC 2001 2012-08-13 2001-01-01
dc.description.abstract <p>One major problem that has high economic impact on pig reproduction is the unexplained loss of potential porcine conceptuses during the first month of gestation. To better understand when and how these losses occur, it is imperative to investigate the underlying genetic regulatory mechanisms. We have recently initiated a large-scale cDNA sequencing project to provide molecular information regarding the genes expressed in female reproductive tissues. cDNA libraries are planned for ovary, hypothalamus, pituitary, placenta, uterus, and several stages of embryonic development. Sequence information will also be highly useful in developing sequence-tagged sites for physical mapping and developing comparative links between the human, mouse, and pig genome maps. We have previously reported the creation of two cDNA libraries, porcine fetal (day 20), and conceptus (day 17). Sequencing of these libraries produced 220 Expressed Sequence Tags (ESTs), with 180 sequences analyzed by clustering algorithms, and 139 clusters identified within these sequences. We now report the creation of two more libraries from porcine fetal (day 45) and placental tissues. The day 45 fetal library has 971,150 independent clones (average insert: 1.4 kb), whereas the placental library has 1,320,000 independent clones. Initial sequencing of the fetal library has produced 119 ESTs (81 clusters), whereas we have obtained 1411 ESTs (1056 clusters) from the placental library. After clustering all sequences thus far obtained, we have identified 1,233 unique clusters. Sequences obtained in this project will be deposited into Genbank dbEST, and all comparative homology</p>
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dc.identifier archive/
dc.identifier.articleid 1024
dc.identifier.contextkey 3210301
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath swinereports_2000/17
dc.language.iso en
dc.relation.ispartofseries Animal Science Research Reports
dc.relation.ispartofseries ASL R668
dc.source.bitstream archive/|||Fri Jan 14 21:09:06 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.keywords ASL R668
dc.title Development of New Placental and Fetal Expressed Sequence Tags (EST) for Gene Discovery in Pig Reproduction
dc.type article
dc.type.genre breeding_physiology
dspace.entity.type Publication
relation.isSeriesOfPublication 7f3839b7-b833-4418-a6fa-adda2b23950a
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