Distribution of type X collagen in tibiotarsi of broiler chickens with vitamin D deficiency

dc.contributor.author Haynes, Joseph
dc.contributor.author Haynes, Joseph
dc.contributor.author Goff, Jesse
dc.contributor.department Veterinary Pathology
dc.date 2018-02-19T00:01:01.000
dc.date.accessioned 2020-07-07T05:15:40Z
dc.date.available 2020-07-07T05:15:40Z
dc.date.issued 1991-05-01
dc.description.abstract <p>Type X collagen is a significant component of the extracellular matrix of the hypertrophic zone of physeal cartilage, but its precise role in endochondral ossification has not been determined. The concentration of type X collagen increases in physeal cartilage in chicks with vitamin D deficiency. The purpose of our study was to determine whether defective endochondral ossification due to vitamin D deficiency was associated with abnormalities in the distribution of type X collagen in the proximal tibiotarsus of chicks. To accomplish this, we induced vitamin D deficiency in broiler chicks and sequentially evaluated the pattern of type X collagen immunoreactivity in the proximal tibiotarsus using a monoclonal antibody specific for chicken type X collagen. Type X collagen immunoreactivity was present in the matrix of the prehypertrophic zone, hypertrophic zone, cartilage cores of the primary spongiosa, and within the chondrocytes of the prehypertrophic and early hypertrophic zones in vitamin D-deficient and D-replete chicks. However, rachitic chicks exhibited two consistent differences in type X collagen immunoreactivity: hypertrophic chondrocytes in the late hypertrophic zone and primary spongiosa contained intracellular type X collagen; and type X collagen was concentrated into laminated aggregates in the pericellular and territorial matrices in the late hypertrophic zone and primary spongiosa. We conclude from these findings that (1) normal serum concentrations of 1,25(OHD)2D3 and 25OHD3 are not required for type X collagen production; (2) type X collagen production does not decrease in the most mature zones of the physes in chicks with vitamin D deficiency; and (3) newly secreted type X collagen accumulates in the pericellular and territorial matrices of the late hypertrophic zone and primary spongiosa of rachitic chicks, perhaps because it is not readily incorporated into the interterritorial matrix.</p>
dc.description.comments <p>This article is published as Haynes, Joseph S., and Jesse Goff. "Distribution of type X collagen in tibiotarsi of broiler chickens with vitamin D deficiency." Calcified tissue international 49, no. 3 (1991): 188-192. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/100/
dc.identifier.articleid 1103
dc.identifier.contextkey 10920336
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/100
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92424
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/100/1991_Haynes_DistributionType.pdf|||Fri Jan 14 18:11:49 UTC 2022
dc.subject.disciplines Poultry or Avian Science
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords type X collagen
dc.subject.keywords Rickets
dc.title Distribution of type X collagen in tibiotarsi of broiler chickens with vitamin D deficiency
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 8c426a1b-1e25-4efc-9283-518a1a6522ed
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
File
Original bundle
Now showing 1 - 1 of 1
Name:
1991_Haynes_DistributionType.pdf
Size:
1.32 MB
Format:
Adobe Portable Document Format
Description:
Collections