Neonatal Ovine Pulmonary Dendritic Cells Support Bovine Respiratory Syncytial Virus Replication with Enhanced Interleukin (IL)-4 And IL-10 Gene Transcripts

dc.contributor.author Ackermann, Mark
dc.contributor.author Fach, Sasha
dc.contributor.author Meyerholz, David
dc.contributor.author Gallup, Jack
dc.contributor.author Gallup, Jack
dc.contributor.author Ackermann, Mark
dc.contributor.author Lehmkuhl, Howard
dc.contributor.author Sacco, Randy
dc.contributor.department Veterinary Pathology
dc.date 2018-02-13T06:19:30.000
dc.date.accessioned 2020-07-07T05:15:40Z
dc.date.available 2020-07-07T05:15:40Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2007
dc.date.embargo 2013-02-25
dc.date.issued 2007-04-10
dc.description.abstract <p>The lung microenvironment is constantly exposed to microorganisms and particulate matter. Lung dendritic cells (DCs) play a crucial role in the uptake and processing of antigens found within the respiratory tract. Respiratory syncytial virus (RSV) is a common respiratory tract pathogen in children that induces an influx of DCs to the mucosal surfaces of the lung. Using a neonatal lamb model, we examined the in vivo permissiveness of DCs to RSV infection, as well as overall cell surface changes and cytokine responses of isolated lung DCs after bovine RSV (BRSV) infection. We report that isolated lung DCs and alveolar macrophages support BRSV replication. Isolated lung DCs were determined to be susceptible to BRSV infection as demonstrated by quantification of BRSV non-structural protein 2 mRNA. BRSV infection induced an initial upregulation of CD14 expression on lung DCs, but by 5 d postinfection expression was similar to that on control cells. No significant changes in CD80/86 or MHC class I expression were seen on lung DCs after BRSV infection. Low to moderate expression of MHC class II and DEC-205 was detected by day 5 postinfection. Initially, on day 3 postinfection, lung DCs from BRSV-infected lambs had decreased endocytosis of fluorescein isothiocyanate (FITC)–ovalbumin (OVA). The amount of FITC–OVA endocytosed by lung DCs isolated on day 5 postinfection was similar to that of controls. The most interesting observation was the induction of immunomodulatory interleukin (IL)-4 and IL-10 cytokine gene transcription in lung DCs and alveolar macrophages after in vivo infection with BRSV. Overall, these findings are the first to demonstrate that neonatal lung DCs support in vivo BRSV replication and produce type II cytokines after viral infection.</p>
dc.description.comments <p>This article is from <em>Viral Immunology </em>20, no. 1 (Spring 2007): 119–130, doi:<a href="http://dx.doi.org/10.1089/vim.2006.0056" target="_blank">10.1089/vim.2006.0056</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/10/
dc.identifier.articleid 1006
dc.identifier.contextkey 3783311
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/10
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92423
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/10/2007_Fach_NeonatalOvinePulmonary.pdf|||Fri Jan 14 18:03:47 UTC 2022
dc.source.uri 10.1089/vim.2006.0056
dc.subject.disciplines Immunology and Infectious Disease
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.title Neonatal Ovine Pulmonary Dendritic Cells Support Bovine Respiratory Syncytial Virus Replication with Enhanced Interleukin (IL)-4 And IL-10 Gene Transcripts
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isAuthorOfPublication ef246218-d0a8-4526-8d8b-4fac40c21ae7
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
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