Fingerloop activates cargo delivery and unloading during cotranslational protein targeting

dc.contributor.author Ariosa, Aileen
dc.contributor.author Duncan, Stacy
dc.contributor.author Saraogi, Oshu
dc.contributor.author Lu, Xiaodong
dc.contributor.author Brown, April
dc.contributor.author Phillips, Gregory
dc.contributor.author Shan, Shu-Ou
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.department Bioinformatics and Computational Biology
dc.date 2018-02-17T06:38:22.000
dc.date.accessioned 2020-07-07T05:15:33Z
dc.date.available 2020-07-07T05:15:33Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.issued 2013-01-15
dc.description.abstract <p>During cotranslational protein targeting by the signal recognition particle (SRP), information about signal sequence binding in the SRP's M domain must be effectively communicated to its GTPase domain to turn on its interaction with the SRP receptor (SR) and thus deliver the cargo proteins to the membrane. A universally conserved “fingerloop” lines the signal sequence–binding groove of SRP; the precise role of this fingerloop in protein targeting has remained elusive. In this study, we show that the fingerloop plays important roles in SRP function by helping to induce the SRP into a more active conformation that facilitates multiple steps in the pathway, including efficient recruitment of SR, GTPase activation in the SRP•SR complex, and most significantly, the unloading of cargo onto the target membrane. On the basis of these results and recent structural work, we propose that the fingerloop is the first structural element to detect signal sequence binding; this information is relayed to the linker connecting the SRP's M and G domains and thus activates the SRP and SR for carrying out downstream steps in the pathway.</p>
dc.description.comments <p>This article is from <em>Molecular Biology of the Cell</em> 24 (2013): 63–73, doi:<a href="http://dx.doi.org/10.1091/mbc.E12-06-0434" target="_blank">10.1091/mbc.E12-06-0434</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/96/
dc.identifier.articleid 1095
dc.identifier.contextkey 7876820
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/96
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92406
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/96/2013_Ariosa_FingerloopActivates.pdf|||Sat Jan 15 02:35:13 UTC 2022
dc.source.uri 10.1091/mbc.E12-06-0434
dc.subject.disciplines Bioinformatics
dc.subject.disciplines Computational Biology
dc.subject.disciplines Molecular Biology
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.title Fingerloop activates cargo delivery and unloading during cotranslational protein targeting
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
relation.isOrgUnitOfPublication c331f825-0643-499a-9eeb-592c7b43b1f5
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