Improved detection of mcyA genes and their phylogenetic origins in harmful algal blooms

dc.contributor.author Lee, Jaejin
dc.contributor.author Choi, Jinlyung
dc.contributor.author Fatka, Micah
dc.contributor.author Swanner, Elizabeth
dc.contributor.author Ikuma, Kaoru
dc.contributor.author Liang, Xuewei
dc.contributor.author Leung, Tania
dc.contributor.author Howe, Adina
dc.contributor.department Geological and Atmospheric Sciences
dc.contributor.department Bioinformatics and Computational Biology
dc.contributor.department Agricultural and Biosystems Engineering
dc.contributor.department Agricultural and Biosystems Engineering
dc.contributor.department Civil, Construction and Environmental Engineering
dc.contributor.department Bioinformatics and Computational Biology
dc.date 2020-03-27T22:19:17.000
dc.date.accessioned 2020-06-29T22:37:02Z
dc.date.available 2020-06-29T22:37:02Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2020
dc.date.embargo 2022-03-19
dc.date.issued 2020-03-19
dc.description.abstract <p>Microcystins, a group of cyanotoxins produced by cyanobacterial strains, have become a significant microbial hazard to human and animal health due to increases in the frequency and intensity of cyanobacterial harmful algal blooms (CyanoHABs). Many studies have explored the correlation between microcystin concentrations and abundances of toxin-producing genes (e.g., <em>mcyA</em> genes) measured using quantitative PCR, and discrepancies between toxin concentrations and gene abundances are often observed. In this study, the results show that these discrepancies are at least partially due to primer sets that do not capture the phylogenetic diversity of naturally present toxin-producers. We designed three novel primer gene probes based on known <em>mcy</em>A genes to improve the detection and quantification of these genes in environmental samples. These primers were shown to improve the identification of <em>mcy</em>A genes compared to previously published primers in freshwater metagenomes, cyanobacterial isolates, and lake water samples. Unlike previously published primers, our primer sets could selectively amplify and resolve <em>Microcystis</em>, <em>Anabaena</em>, and <em>Planktothrix mcy</em>A genes. In lake water samples, abundance estimations of <em>mcy</em>A genes were found to correlate strongly with microcystin concentrations. Based on our results, these primers offer significant improvements over previously published probes to accurately identify and quantify <em>mcy</em>A genes in the environment. There is an increasing need to develop models based on microbial information and environmental factors to predict CyanoHABs, and improved primers will play an important role in aiding monitoring efforts to collect reliable and consistent data on toxicity risks.</p>
dc.description.comments <p>This is a manuscript of an article published as Lee, Jaejin, Jinlyung Choi, Micah Fatka, Elizabeth Swanner, Kaoru Ikuma, Xuewei Liang, Tania Leung, and Adina Howe. "Improved detection of mcyA genes and their phylogenetic origins in harmful algal blooms." <em>Water Research</em> (2020): 115730. DOI: <a href="https://doi.org/10.1016/j.watres.2020.115730" target="_blank">10.1016/j.watres.2020.115730</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/abe_eng_pubs/1120/
dc.identifier.articleid 2405
dc.identifier.contextkey 17125060
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath abe_eng_pubs/1120
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/824
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/abe_eng_pubs/1120/2020_HoweAdina_ImprovedDetection.pdf|||Fri Jan 14 18:45:06 UTC 2022
dc.source.uri 10.1016/j.watres.2020.115730
dc.subject.disciplines Bioresource and Agricultural Engineering
dc.subject.disciplines Environmental Microbiology and Microbial Ecology
dc.subject.disciplines Water Resource Management
dc.subject.keywords Cyanobacterial harmful algal blooms
dc.subject.keywords Microcystins
dc.subject.keywords mcyA gene
dc.subject.keywords Primer design
dc.subject.keywords Quantitative PCR
dc.title Improved detection of mcyA genes and their phylogenetic origins in harmful algal blooms
dc.type article
dc.type.genre article
dspace.entity.type Publication
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