Characterization of a cytosolic acyl-activating enzyme catalyzing the formation of 4-methylvaleryl-CoA for pogostone biosynthesis in Pogostemon cablin

Date
2021-10
Authors
Chen, Jing
Liu, Lang
Wang, Ying
Li, Zhengguo
Wang, Guodong
Kraus, George
Pichersky, Eran
Xu, Haiyang
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Oxford University Press
Altmetrics
Authors
Research Projects
Organizational Units
Chemistry
Organizational Unit
Ames Laboratory
Organizational Unit
Journal Issue
Series
Department
ChemistryAmes Laboratory
Abstract
Pogostone, a compound with various pharmaceutical activities, is a major constituent of the essential oil preparation called Pogostemonis Herba, which is obtained from the plant Pogostemon cablin. The biosynthesis of pogostone has not been elucidated, but 4-methylvaleryl-CoA (4MVCoA) is a likely precursor. We analyzed the distribution of pogostone in P. cablin using gas chromatography-mass spectrometry (GC-MS) and found that pogostone accumulates at high levels in the main stems and leaves of young plants. A search for the acyl-activating enzyme (AAE) that catalyzes the formation of 4MVCoA from 4-methylvaleric acid was launched, using an RNAseq-based approach to identify 31 unigenes encoding putative AAEs including the PcAAE2, the transcript profile of which shows a strong positive correlation with the distribution pattern of pogostone. The protein encoded by PcAAE2 was biochemically characterized in vitro and shown to catalyze the formation of 4MVCoA from 4-methylvaleric acid. Phylogenetic analysis showed that PcAAE2 is closely related to other AAE proteins in P. cablin and other species that are localized to the peroxisomes. However, PcAAE2 lacks a peroxisome targeting sequence 1 (PTS1) and is localized in the cytosol.
Comments
This article is published as Chen, Jing, Lang Liu, Ying Wang, Zhengguo Li, Guodong Wang, George A. Kraus, Eran Pichersky, and Haiyang Xu. "Characterization of a Cytosolic Acyl-Activating Enzyme Catalyzing the Formation of 4-Methylvaleryl-CoA for Pogostone Biosynthesis in Pogostemon Cablin." Plant and Cell Physiology 62, no. 10 (2021): 1556-1571. DOI: 10.1093/pcp/pcab111. Copyright 2021 The Author(s). Attribution 4.0 International (CC BY 4.0). Posted with permission.
Description
Keywords
Citation
DOI
Collections