Isolation and Characterization of a Canine Corneal Epithelial Cell Population

dc.contributor.author Ludwig, Allison
dc.contributor.department Veterinary Clinical Sciences
dc.date 2018-02-14T09:55:20.000
dc.date.accessioned 2020-07-07T05:10:41Z
dc.date.available 2020-07-07T05:10:41Z
dc.date.issued 2014-04-15
dc.description.abstract <p>The use of corneal epithelial cells (CECs) for evaluation of the effect of different treatment modalities on corneal healing is an important technique that reduces the need for experiments in live animals. The isolation and culture of corneal epithelial cells is often a difficult process due to the structure of the cornea and the proliferative nature of corneal keratocytes. This project was focused on the isolation, culture, and characterization of a population of canine CECs as a first step toward use in future studies of the effects of treatment on the healing of CECs defects. In this study, we isolated a population of cells from canine corneas, grew the cells in culture for several passages, and evaluated the cells for morphological characteristics of CECs. Immunostaining for cytokeratin and vimentin was conducted to verify the epithelial nature of the isolated cells, and data regarding the percentage of CECs present in each population was recorded. Our future studies will be aimed at evaluation of different medical treatments on the proliferation of isolated CECs and healing of canine corneal defects.</p>
dc.identifier archive/lib.dr.iastate.edu/undergradresearch_symposium/2014/presentations/6/
dc.identifier.articleid 1005
dc.identifier.contextkey 5914564
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath undergradresearch_symposium/2014/presentations/6
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91625
dc.relation.ispartofseries Symposium on Undergraduate Research and Creative Expression
dc.source.bitstream archive/lib.dr.iastate.edu/undergradresearch_symposium/2014/presentations/6/LudwigAllisonI.pdf|||Sat Jan 15 01:05:51 UTC 2022
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Isolation and Characterization of a Canine Corneal Epithelial Cell Population
dc.type event
dc.type.genre article
dspace.entity.type Publication
relation.isOrgUnitOfPublication 1ad68def-86ae-460b-8808-f1b1febafd0a
relation.isSeriesOfPublication 6730f354-97b8-4408-bad3-7e5c3b2fca9d
thesis.degree.discipline Animal Science
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