A destoner fractionation process was used to separate distillers dried grains with solubles (DDGS) into streams with various compositions. Results showed that destoner fractionation was somewhat efficient and effective. Runs with 8° angle and 27.5 percent air flow resulted in the highest value of protein and oil, which the light fraction had 28.15% protein, 10.50% oil, while the heavy fraction had 31.30% protein and 17.20% oil. Particle size distribution had a positive correlation coefficient (0.93) with oil parameters and a negative correlation coefficient (-0.96) with moisture parameters. Fiber had no relationship with particle size, and protein had a weak correlation coefficient with (-0.54) to particle size.