Behavior of Neural Cells Post Manufacturing and After Prolonged Encapsulation within Conductive Graphene-Laden Alginate Microfibers

dc.contributor.author McNamara, Marilyn C.
dc.contributor.author Aykar, Saurabh S.
dc.contributor.author Alimoradi, Nima
dc.contributor.author Niaraki Asli, Amir Ehsan
dc.contributor.author Pemathilaka, Rajeendra L.
dc.contributor.author Wrede, Alex H.
dc.contributor.author Montazami, Reza
dc.contributor.other Mechanical Engineering
dc.contributor.other Biomedical Sciences
dc.contributor.other Ames Laboratory
dc.contributor.other Bioeconomy Institute
dc.date.accessioned 2022-01-05T16:34:03Z
dc.date.available 2022-01-05T16:34:03Z
dc.date.issued 2021-11
dc.description.abstract Engineering conductive 3D cell scaffoldings offer advantages toward the creation of physiologically relevant platforms with integrated real-time sensing capabilities. Dopaminergic neural cells are encapsulated into graphene-laden alginate microfibers using a microfluidic approach, which is unmatched for creating highly-tunable microfibers. Incorporating graphene increases the conductivity of the alginate microfibers by 148%, creating a similar conductivity to native brain tissue. The cell encapsulation procedure has an efficiency of 50%, and of those cells, ≈30% remain for the entire 6-day observation period. To understand how the microfluidic encapsulation affects cell genetics, tyrosine hydroxylase, tubulin beta 3 class 3, interleukin 1 beta, and tumor necrosis factor alfa are analyzed primarily with real-time reverse transcription-quantitative polymerase chain reaction and secondarily with enzyme-linked immunosorbent assay, immediately after manufacturing, after encapsulation in polymer matrix for 6 days, and after encapsulation in the graphene-polymer composite for 6 days. Preliminary data shows that the manufacturing process and combination with alginate matrix affect the expression of the studied genes immediately after manufacturing. In addition, the introduction of graphene further changes gene expressions. Long-term encapsulation of neural cells in alginate and 6-day exposure to graphene also leads to changes in gene expressions.
dc.description.comments This is the published version of the following article: McNamara, Marilyn C., Saurabh S. Aykar, Nima Alimoradi, Amir Ehsan Niaraki Asli, Rajeendra L. Pemathilaka, Alex H. Wrede, Reza Montazami, and Nicole Hashemi. "Behavior of Neural Cells Post Manufacturing and After Prolonged Encapsulation within Conductive Graphene-Laden Alginate Microfibers." Advanced Biology 5, no. 11 (2021): 2101026. DOI: 10.1002/adbi.202101026. Copyright 2021 The Authors. Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). Posted with permission.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/7vdXl6Gv
dc.language.iso en_US
dc.publisher Wiley-VCH GmbH
dc.source.uri https://doi.org/10.1002/adbi.202101026 *
dc.subject neural cells
dc.subject alginate microfibers
dc.subject graphene
dc.subject qPCR
dc.subject conductive hydrogel
dc.subject.disciplines DegreeDisciplines::Engineering::Mechanical Engineering::Biomechanical Engineering
dc.subject.disciplines DegreeDisciplines::Engineering::Materials Science and Engineering::Biology and Biomimetic Materials
dc.title Behavior of Neural Cells Post Manufacturing and After Prolonged Encapsulation within Conductive Graphene-Laden Alginate Microfibers
dc.type Article
dspace.entity.type Publication
relation.isAuthorOfPublication f1ba9f2a-a64d-43aa-97ca-0d72675c4f2e
relation.isOrgUnitOfPublication 6d38ab0f-8cc2-4ad3-90b1-67a60c5a6f59
File
Original bundle
Now showing 1 - 1 of 1
Name:
2021-HashemiNicole-BehaviorNeural.pdf
Size:
2.84 MB
Format:
Adobe Portable Document Format
Description:
Collections