Vaccination with NS1-Truncated H3N2 Swine Influenza Virus Primes T Cells and Confers Cross-Protection against an H1N1 Heterosubtypic Challenge in Pigs

dc.contributor.author Wang, Chong
dc.contributor.author Kappes, Matthew
dc.contributor.author Sandbulte, Matthew
dc.contributor.author Platt, Ratree
dc.contributor.author Roth, James
dc.contributor.author Wang, Chong
dc.contributor.author Lager, Kelly
dc.contributor.author Henningson, Jamie
dc.contributor.author Lorusso, Alessio
dc.contributor.author Vincent, Amy
dc.contributor.author Loving, Crystal
dc.contributor.author Roth, James
dc.contributor.author Kehrli, Marcus
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-17T04:46:11.000
dc.date.accessioned 2020-07-07T05:15:29Z
dc.date.available 2020-07-07T05:15:29Z
dc.date.issued 2012-01-01
dc.description.abstract <p>The diversity of contemporary swine influenza virus (SIV) strains impedes effective immunization of swine herds. Mucosally delivered, attenuated virus vaccines are one approach with potential to provide broad cross-protection. Reverse genetics-derived H3N2 SIV virus with truncated NS1 (NS1Δ126 TX98) is attenuated and immunogenic when delivered intranasally in young pigs. We analyzed T-cell priming and cross-protective efficacy in weanling piglets after intranasal inoculation with NS1Δ126 TX98 versus wild type TX98. <em>In vivo</em> replication of the truncation mutant was minimal compared to the wild type virus. T-cell responses were greater in magnitude in pigs infected with the wild type virus in <em>in vitro</em> restimulation assays. According to the expression of activation marker CD25, peripheral T cell recall responses in NS1Δ126 TX98 infected pigs were minimal. However, intracellular IFN-γ data indicate that the attenuated virus induced virus-specific CD4<sup>+</sup>CD8<sup>–</sup>, CD4<sup>+</sup>CD8<sup>+</sup>, CD4<sup>–</sup>CD8<sup>+</sup>, and γδ T cells within 28 days. The IFN-γ response appeared to contract, as responses were reduced at later time points prior to challenge. CD4<sup>+</sup>CD8<sup>+</sup> cells isolated 5 days after heterosubtypic H1N1 challenge (day 70 overall) showed an elevated CD25 response to virus restimulation. Pigs previously infected with wild type TX98 were protected from replication of the H1N1 challenge virus. Vaccination with NS1Δ126 TX98 was associated with significantly lower levels of Th1-associated cytokines in infected lungs but provided partial cross-protection against the H1N1 challenge. These results demonstrate that NS1Δ SIV vaccines can elicit cell-mediated cross-protection against antigenically divergent strains.</p>
dc.description.comments <p>This article is from <em>Vaccine</em> 30 (2012): 280, doi:<a href="http://dx.doi.org/10.1016/j.vaccine.2011.10.098" id="x-ddDoi" target="_blank">10.1016/j.vaccine.2011.10.098</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/83/
dc.identifier.articleid 1076
dc.identifier.contextkey 7792650
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/83
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92392
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/83/2012_Roth_VaccinationNS1Truncated.pdf|||Sat Jan 15 02:09:24 UTC 2022
dc.source.uri 10.1016/j.vaccine.2011.10.098
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords Swine influenza virus
dc.subject.keywords Modified live vaccine
dc.subject.keywords T-cell immunity
dc.subject.keywords Heterosubtypic cross protection
dc.title Vaccination with NS1-Truncated H3N2 Swine Influenza Virus Primes T Cells and Confers Cross-Protection against an H1N1 Heterosubtypic Challenge in Pigs
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isAuthorOfPublication 909dd0b2-ec0a-41e2-b4e0-9e5ff76b7622
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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