First molecular detection of hepatitis E virus genome in camel and pig faecal samples in Ethiopia

dc.contributor.author Dawo Bari, Fufa
dc.contributor.author Belete Wodaje, Haimanot
dc.contributor.author Plummer, Paul
dc.contributor.author Said, Umar
dc.contributor.author Waktole, Hika
dc.contributor.author Sombo, Melaku
dc.contributor.author Leta, Samson
dc.contributor.author Rufael Chibsa, Tesfaye
dc.contributor.author Plummer, Paul
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2021-08-09T18:23:35.000
dc.date.accessioned 2021-08-15T02:05:18Z
dc.date.available 2021-08-15T02:05:18Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2021
dc.date.issued 2021-08-04
dc.description.abstract <p>Background: Hepatitis E is an enteric and zoonotic disease caused by hepatitis E virus (HEV) that is mainly transmitted via the faecal-oral route through contaminated food or the environment. The virus is an emerging infectious agent causing acute human infection worldwide. A high seroprevalence of the disease was reported in pregnant women in Addis Ababa, Ethiopia, raising significant public health concern. The presence of HEV specific antibodies were also reported in dromedary camels in the country; however, the infectious virus and/or the viral genome have not been demonstrated to date in animal samples.</p> <p>Methods: To address this gap, a total of 95 faecal samples collected from both apparently healthy pigs of uncharacterised types (50 samples) in Burayu and Addis Ababa areas and camels (Camelus dromedarius, 45 samples) in west Hararghe were screened for the presence of HEV genome using universal primers in a fully nested reverse transcription polymerase chain reaction (nRT-PCR). The protocol is capable of detecting HEV in faecal samples from both pigs and camels.</p> <p>Results: The nRT-PCR detected HEV genes in six (12%) pig faecal samples and one camel sample (2.2%). Therefore, the results indicate that HEV is circulating in both pigs and camels in Ethiopia and these animals and their products could serve as a potential source of infection for humans.</p> <p>Conclusion: The detection of HEV in both animals could raise another concern regarding its public health importance as both animals’ meat and camel milk are consumed in the country. Further studies to determine the prevalence and distribution of the virus in different animals and their products, water bodies, food chain, and vegetables are warranted, along with viral gene sequencing for detailed genetic characterisation of the isolates circulating in the country. This information is critically important to design and institute appropriate control and/or preventive measures.</p>
dc.description.comments <p>This article is published in <em>Virology Journal</em>. DOI: <a href="https://doi.org/10.1186/s12985-021-01626-9" target="_blank">10.1186/s12985-021-01626-9</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/213/
dc.identifier.articleid 1217
dc.identifier.contextkey 24248982
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/213
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/jrl87yJr
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/213/2021_PlummerPaul_FirstMolecular.pdf|||Fri Jan 14 22:36:28 UTC 2022
dc.source.uri 10.1186/s12985-021-01626-9
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title First molecular detection of hepatitis E virus genome in camel and pig faecal samples in Ethiopia
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication f16d5130-7036-437d-892d-b4a4072903e7
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
File
Original bundle
Now showing 1 - 1 of 1
Name:
2021_PlummerPaul_FirstMolecular.pdf
Size:
951.81 KB
Format:
Adobe Portable Document Format
Description:
Collections