Comparison of humoral and T-cell-mediated immune responses to a single dose of Bovela® live double deleted BVDV vaccine or to a field BVDV strain Wang, Chong Platt, Ratree Kesl, Lyle Guidarini, Christian Wang, Chong Roth, James Roth, James
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine 2018-02-18T08:18:03.000 2020-07-07T05:14:30Z 2020-07-07T05:14:30Z Sun Jan 01 00:00:00 UTC 2017 2017-05-01
dc.description.abstract <p>The objective of this study was to determine and compare the humoral and cellular immune responses of calves exposed to a single dose of Bovela<sup>®</sup> bovine viral diarrhea virus (BVDV) live double deleted vaccine or a field strain virus (FSV) of BVDV type 2 (strain 890). Thirty seronegative, colostrum-deprived 5 month-old Holstein steer calves that tested negative for persistent BVDV by ear notch immunohistochemistry and seronegative to BVDV types 1 and 2 were used. Calves were screened by multi-parameter flow cytometry (MP-FCM) 1 week before vaccination to ensure that they were negative for T cell responses to the BVDV types 1 and 2 viruses in the Bovela<sup>®</sup> vaccine. Calves were assigned to 3 treatment groups: control (PBS), FSV inoculated, and Bovela<sup>®</sup> vaccinated. The humoral response was tested by standard serum virus neutralization (SVN) test to BVDV types 1 (Singer strain) and 2 (strain 125). The response by CD4, CD8, and gamma delta (γδ TCR) T cells was evaluated by MP-FCM using individual BVDV types 1 and 2 from Bovela<sup>®</sup> vaccine as recall antigens at 5, 6, and 7 weeks after vaccination. Activation markers used were upregulation of surface CD25 (IL-2R), intracellular interferon gamma (IFNγ) and intracellular interleukin 4 (IL-4). Each T cell subset was evaluated for increased expression of each activation marker compared to non-antigen stimulated cells of the same animal. All Bovela<sup>®</sup> vaccinated and FSV inoculated calves produced SVN antibodies to both BVDV types 1 and 2 while control animals remained seronegative throughout the study. The mean (weeks 5, 6, and 7) T cell recall responses to Bovela<sup>®</sup> BVDV type 1 and type 2 recall antigens were numerically higher in all three T cell subsets (CD4, CD8, and γδ TCR) for all three activation markers (CD25, IFNγ, and IL-4) when compared to either the control animals or to the FSV inoculated animals. These differences were often, but not always, statistically significant (P < 0.05)</p>
dc.description.comments <p>This article is from <em>Veterinary Immunology and Immunopathology </em>187 (2017): 20, doi: <a href="" id="x-ddDoi">10.1016/j.vetimm.2017.03.003</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 1134
dc.identifier.contextkey 9971792
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/135
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 19:54:14 UTC 2022
dc.source.uri 10.1016/j.vetimm.2017.03.003
dc.subject.disciplines Comparative and Laboratory Animal Medicine
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Comparison of humoral and T-cell-mediated immune responses to a single dose of Bovela® live double deleted BVDV vaccine or to a field BVDV strain
dc.type article
dc.type.genre article
dspace.entity.type Publication
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