Aptasensor based on specific binding induced impedance changes in nanoporous anodized alumina membranes

dc.contributor.author Jiang, Nianyu
dc.contributor.author Banerjee, Soma
dc.contributor.author Nilsen-Hamilton, Marit
dc.contributor.author Shrotriya, Pranav
dc.contributor.department Mechanical Engineering
dc.contributor.department Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of
dc.date 2021-05-10T17:55:25.000
dc.date.accessioned 2021-08-14T19:06:25Z
dc.date.available 2021-08-14T19:06:25Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2021
dc.date.embargo 2020-01-01
dc.date.issued 2021-05-05
dc.description.abstract <p>Monitoring electrochemical impedance changes due to aptamer/ligand binding on nanochannel surface provides an attractive sensing mechanism for chemical and biological species. We report a surface charge modulation based sensing mechanism for the detection of small molecules – amodiaquine and homoserine lactone with their respective specifically binding aptamers. The change in charge distribution due to aptamer/ligand binding modifies ionic transport across the nanochannel, results in transmembrane impedance changes proportional to the amount of target present, providing a quantitative response. A sensor reader based on an analog devices chip ADuCM355 was developed and used to monitor the nanoporous membrane's impedance changes over the desired frequency range. These results show that the combination of a low cost sensor reader and aptamer functionalized membrane will enable the development of a portable and inexpensive sensor system.</p>
dc.description.comments <p>This presentation is published as Nianyu Jiang, Soma Banerjee, Marit Nilsen-Hamilton, and Pranav Shrotriya. "Aptasensor based on specific binding induced impedance changes in nanoporous anodized alumina membranes," in <em>Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXII</em>, edited by Jason A. Guicheteau, Chris R. Howle, Proc. of SPIE 11749, 117490H (12 April 2021); DOI: <a href="https://doi.org/10.1117/12.2586170" target="_blank">10.1117/12.2586170</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/me_conf/208/
dc.identifier.articleid 1207
dc.identifier.contextkey 22821186
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath me_conf/208
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/5w5py6xz
dc.language.iso en
dc.source.uri https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11749/117490H/Aptasensor-based-on-specific-binding-induced-impedance-changes-in-nanoporous/10.1117/12.2586170.full
dc.subject.disciplines Biochemical and Biomolecular Engineering
dc.subject.disciplines Molecular, Cellular, and Tissue Engineering
dc.title Aptasensor based on specific binding induced impedance changes in nanoporous anodized alumina membranes
dc.type article
dc.type.genre presentation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 6d38ab0f-8cc2-4ad3-90b1-67a60c5a6f59