For sweat glands, it has been shown that sympathetic neurons express a cholinergic/noradrenergic co-phenotype in their innervation in the trunk of mice.(Schütz et al. 2008). It is unknown if facial sweat glands are innervated the same way. Gustatory sweating is an abnormal sweating condition. People can sweat profusely when eating or drinking, or even thinking about eating or drinking. We hypothesize that the facial sweat glands are controlled differently and can be related to parasympathetic neurons from cranial nerves. Some samples from human donors have been tested, but few sweat glands can be targeted since the donors were older people. Thus, it was decided to use horse tissue for this project to obtain greater numbers of sweat glands on the face. A challenge for this project included a lack of availability of an equine-specific antibody to label vesicular ACh transporter. A successful antibody for tyrosine hydroxylase was identified for horse tissue but the staining results suggest a lack of specificity for tyrosine hydroxylase. This demonstrates a need for a more specific antibody in horse to determine actual labeling of tyrosine hydroxylase. In addition, increase numbers of samples and slide preparations may improve the ability to identify properly label tyrosine hydroxylase.