Enzymatic Assay Method for Evaluating the Lipase Activity in Complex Extracts from Transgenic Corn Seed

dc.contributor.author Zhong, Qixin
dc.contributor.author Glatz, Charles
dc.contributor.author Glatz, Charles
dc.contributor.department Chemical and Biological Engineering
dc.date 2018-02-13T21:14:04.000
dc.date.accessioned 2020-06-30T01:10:47Z
dc.date.available 2020-06-30T01:10:47Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2006
dc.date.embargo 2014-02-11
dc.date.issued 2006-05-03
dc.description.abstract <p>A colorimetric method was established to determine the activity of recombinant lipase in extracts from transgenic corn seed. The system was an oil-in-water emulsion that was stabilized by a surfactant to accommodate the organic phase substrate and aqueous phase enzyme. The lipase activity was measured by monitoring the release of nitrophenol at 346 nm from the substrate, 4-nitrophenyl butyrate. Emulsions prepared with various surfactant types and concentrations were tested. For each surfactant, the measured activity was greatest when the surfactant concentration was close to the critical micelle concentration, consistent with the changing trend of oil droplet size as a function of surfactant concentration. The optimal system, with 0.01% (w/w) Tween 80, demonstrated good reproducibility, high sensitivity, robustness, and a linear response to lipase concentration.</p>
dc.description.comments <p>Reprinted with permission from <em>Journal of Agricultural and Food Chemistry</em> 54 (2006): 3181–3185, doi:<a href="http://dx.doi.org/10.1021/jf052016k" target="_blank">10.1021/jf052016k</a>, Copyright 2006 American Chemical Society.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/cbe_pubs/76/
dc.identifier.articleid 1075
dc.identifier.contextkey 5099500
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cbe_pubs/76
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/13577
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/76/2006_ZhongQ_EnzymaticAssayMethod.pdf|||Sat Jan 15 01:50:36 UTC 2022
dc.source.uri 10.1021/jf052016k
dc.subject.disciplines Biochemical and Biomolecular Engineering
dc.subject.disciplines Biological Engineering
dc.subject.keywords Enzyme assay
dc.subject.keywords lipase
dc.subject.keywords emulsion
dc.title Enzymatic Assay Method for Evaluating the Lipase Activity in Complex Extracts from Transgenic Corn Seed
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 0005bc3a-6553-4c02-9985-c97edd4719fc
relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
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