Proteolytic Cleavage of the Ectodomain of the L1 CAM Family Member Tractin

dc.contributor.author Xu, Ying-Zhi
dc.contributor.author Johansen, Kristen
dc.contributor.author Ji, Yun
dc.contributor.author Zipser, Birgit
dc.contributor.author Jellies, John
dc.contributor.author Johansen, Kristen
dc.contributor.author Johansen, Jorgen
dc.contributor.department Zoology and Genetics
dc.date 2019-07-09T02:30:42.000
dc.date.accessioned 2020-07-07T05:17:00Z
dc.date.available 2020-07-07T05:17:00Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2003
dc.date.issued 2003-02-07
dc.description.abstract <p>Tractin is a member of the L1 family of cell adhesion molecules in leech. Immunoblot analysis suggests that Tractin is constitutively cleaved <em>in vivo</em> at a proteolytic site with the sequence RKRRSR. This sequence conforms to the consensus sequence for cleavage by members of the furin family of convertases, and this proteolytic site is shared by a majority of other L1 family members. We provide evidence with furin-specific inhibitor experiments, by site-specific mutagenesis of Tractin constructs expressed in S2 cells, as well as by Tractin expression in furin-deficient LoVo cells that a furin convertase is the likely protease mediating this processing. Cross-immunoprecipitations with Tractin domain-specific antibodies suggest that the resulting NH<sub>2</sub>- and COOH-terminal cleavage fragments interact with each other and that this interaction provides a means for the NH<sub>2</sub>-terminal fragment to be tethered to the membrane. Furthermore, in S2 cell aggregation assays we show that the NH<sub>2</sub>-terminal fragment is necessary for homophilic adhesion and that cells expressing only the transmembrane COOH-terminal fragment are non-adhesive. However, tethering of exogeneously provided Tractin NH<sub>2</sub>-terminal fragment to S2 cells expressing only the COOH-terminal fragment can functionally restore the adhesive properties of Tractin.</p>
dc.description.comments <p>This article is published as Xu, Ying-Zhi, Yun Ji, Birgit Zipser, John Jellies, Kristen M. Johansen, and Jørgen Johansen. "Proteolytic cleavage of the ectodomain of the L1 CAM family member Tractin." <em>Journal of Biological Chemistry</em> 278, no. 6 (2003): 4322-4330. doi: <a href="http://dx.doi.org/10.1074/jbc.M210775200" target="_blank">10.1074/jbc.M210775200</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/zool_pubs/50/
dc.identifier.articleid 1052
dc.identifier.contextkey 14515135
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath zool_pubs/50
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92662
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/zool_pubs/50/2003_Johansen_ProteolyticCleavage.pdf|||Sat Jan 15 00:41:23 UTC 2022
dc.source.uri 10.1074/jbc.M210775200
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Molecular Biology
dc.title Proteolytic Cleavage of the Ectodomain of the L1 CAM Family Member Tractin
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 3829d759-f8ec-4502-a63a-1655d3b70ef5
relation.isOrgUnitOfPublication 4a2929da-5374-4338-b62f-f5fd9e156ef9
File
Original bundle
Now showing 1 - 1 of 1
Name:
2003_Johansen_ProteolyticCleavage.pdf
Size:
516.76 KB
Format:
Adobe Portable Document Format
Description:
Collections