Software to simulate genomic selection

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2017-04-11
Authors
Shibamoto, Takao
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Altmetrics
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Industrial and Manufacturing Systems Engineering
Abstract

We studied the effects of Activator (Ac) transposon (a chromosomal segment that can undergo transposition) in the development of twin sectors on maize ears containing the allele P1-ovov454. This allele conditions orange-variegated pericarp and cob due to the presence of the Ac transposon in the p1 gene which controls kernel pericarp color. We screened for ears with multi-kernel dark red/orange sectors twinned with sectors of colorless pericarp. From this visual screen, we identified 6 different twin sectors on two maize ears. A series of screening PCR tests were developed to classify the type of structural changes present in each case. Then the junctions of the Ac transposon and flaking DNA were amplified by PCR and sequenced. Based on the results, we determined the structure of each new allele and proposed a mechanism of origin for most cases. Some were formed by simple Ac transposition, while others were generated by more complex recombination events.

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