Microfluidics-enabled method to identify modes of Caenorhabditis elegans paralysis in four anthelmintics

Lycke, Roy
Parashar, Archana
Pandey, Santosh
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AIP Publishing LLC
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Electrical and Computer Engineering
The discovery of new drugs is often propelled by the increasing resistance of parasites to existing drugs and the availability of better technology platforms. The area of microfluidics has provided devices for faster screening of compounds, controlled sampling/sorting of whole animals, and automated behavioral pattern recognition. In most microfluidic devices, drug effects on small animals (e.g., Caenorhabditis elegans) are quantified by an end-point, dose response curve representing a single parameter (such as worm velocity or stroke frequency). Here, we present a multi-parameter extraction method to characterize modes of paralysis in C. elegans over an extended time period. A microfluidic device with real-time imaging is used to expose C. elegans to four anthelmintic drugs (i.e., pyrantel, levamisole, tribendimidine, and methyridine). We quantified worm behavior with parameters such as curls per second, types of paralyzation, mode frequency, and number/duration of active/immobilization periods. Each drug was chosen at EC75 where 75% of the worm population is responsive to the drug. At equipotent concentrations, we observed differences in the manner with which worms paralyzed in drug environments. Our study highlights the need for assaying drug effects on small animal models with multiple parameters quantified at regular time points over an extended period to adequately capture the resistance and adaptability in chemical environments.
This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in Lycke, Roy, Archana Parashar, and Santosh Pandey. "Microfluidics-enabled method to identify modes of Caenorhabditis elegans paralysis in four anthelmintics." Biomicrofluidics 7, no. 6 (2013): 064103, and may be found at DOI: 10.1063/1.4829777. Copyright 2013 AIP Publishing LLC. Posted with permission.