Use of sugarcane mosaic virus for virus-induced gene silencing in maize

dc.contributor.author Whitham, Steven
dc.contributor.author Zhang, Shudi
dc.contributor.author Song, Hojun
dc.contributor.author Whitham, Steven A.
dc.contributor.author Jander, Georg
dc.contributor.department Plant Pathology and Microbiology
dc.date.accessioned 2022-03-03T16:41:02Z
dc.date.available 2022-03-03T16:41:02Z
dc.date.issued 2022-02-20
dc.description.abstract Previously, sugarcane mosaic virus (SCMV) was developed as a vector for transient expression of heterologous genes in Zea mays (maize). Here we show that SCMV can also be applied for virus-induced gene silencing (VIGS) of endogenous maize genes. Comparison of sense and antisense VIGS constructs targeting maize PDS (phytoene desaturase) showed that antisense constructs resulted in a greater reduction in gene expression. In a time course of gene expression after infection with VIGS constructs targeting PDS, Les22 (Lesion mimic 22), and Ij1 (Iodent japonica1), efficient expression silencing was observed two, three, and four weeks after infection with SCMV. However, at week five, expression of Les22 and Ij1 was no longer significantly reduced compared to control plants. The defense signaling molecule jasmonate-isoleucine (JA-Ile) can be inactivated by 12C-hydroxylation and hydrolysis, and knockout of these genes leads to increased herbivore resistance. JA-Ile hydroxylases and hydrolases have been investigated in Arabidopsis, rice, and Nicotiana attenuata. To determine whether the maize homologs of these genes function in plant defense, we silenced expression of ZmCYP94B1 (predicted JA-Ile hydroxylase) and ZmJIH1 (predicted JA-Ile hydrolase) by VIGS with SCMV. Although ZmCYP94B1 and ZmJIH1gene expression silencing increased resistance to Spodoptera frugiperda (fall armyworm), Schistocerca americana (American birdwing grasshopper), and Rhopalosiphum maidis (corn leaf aphid), there was no additive effect from silencing the expression of both genes. Further work will be required to determine the more precise functions of these enzymes in regulating maize defenses.
dc.description.comments This preprint is made available through bioRxiv at doi:10.1101/2022.02.20.481188. It is made available under a CC-BY 4.0 International license.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/EzR2Bj5z
dc.language.iso en
dc.publisher © 2022 The Authors
dc.source.uri https://doi.org/10.1101/2022.02.20.481188 *
dc.subject Sugarcane mosaic virus
dc.subject maize
dc.subject VIGS
dc.subject gene expression silencing
dc.subject jasmonate-isoleucine
dc.subject caterpillar
dc.subject grasshopper
dc.subject aphid
dc.title Use of sugarcane mosaic virus for virus-induced gene silencing in maize
dc.type Preprint
dspace.entity.type Publication
relation.isAuthorOfPublication bd8b6e79-2efb-4611-9a27-13792f2b9a43
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
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