Quantification of the Group B Soyasaponins by High-PerformanceLiquid Chromatography

dc.contributor.author Hu, Jiang
dc.contributor.author Hendrich, Suzanne
dc.contributor.author Lee, Sun-Ok
dc.contributor.author Hendrich, Suzanne
dc.contributor.author Murphy, Patricia
dc.contributor.department Food Science and Human Nutrition
dc.date 2018-02-15T10:44:10.000
dc.date.accessioned 2020-06-30T03:59:56Z
dc.date.available 2020-06-30T03:59:56Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2002
dc.date.embargo 2014-01-28
dc.date.issued 2002-03-26
dc.description.abstract <p>High-performance liquid chromatographic methods were developed for the isolation and quantitative determination of the group B soyasaponins, including 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran- 4-one (DDMP)-conjugated soyasaponins Rg, f3g, and f3a, and their non-DDMP counterparts, soyasaponins V, I, and II, respectively, with formononetin used as the internal standard. The limits of quantification for soy products were 0.11-4.86 µmol/g. The within-day and between-days assay coefficients of variation were <9.8 and < 14.3%, respectively. The group B soyasaponin concentrations in 46 soybean varieties ranged from 2.50 to 5.85 µmol/g. Soy ingredients (soybean flour, toasted soy hypocotyls, soy protein isolates, textured vegetable protein, soy protein concentrates, and Novasoy) and soy foods (commercial soy milk, tofu, and tempeh) contained the group B soyasaponins from 0.20 to 114.02 µmol/g. There was no apparent correlation between isoflavone and soyasaponin concentrations in the soy products examined.</p>
dc.description.comments <p>Reprinted with permission from <em>Journal of Agricultural and Food Chemistry</em>,50(9):2587-2594. doi: <a href="http://www.dx.doi.org/10.1021/jf0114740" target="_blank">10.1021/jf0114740</a>. Copyright 2002 American Chemical Society.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/fshn_ag_pubs/93/
dc.identifier.articleid 1093
dc.identifier.contextkey 6359960
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath fshn_ag_pubs/93
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37608
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/fshn_ag_pubs/93/0-ACS_License_QuantificationGroupB.docx|||Sat Jan 15 02:31:05 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/fshn_ag_pubs/93/2002_HendrichS_QuantificationGroupB.pdf|||Sat Jan 15 02:31:06 UTC 2022
dc.source.uri 10.1021/jf0114740
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Food Science
dc.subject.disciplines Human and Clinical Nutrition
dc.subject.keywords Soyasaponin
dc.subject.keywords isoflavone
dc.subject.keywords soybean foods
dc.title Quantification of the Group B Soyasaponins by High-PerformanceLiquid Chromatography
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 6a3e3a31-206e-45c1-b181-8f1ae5931bf5
relation.isOrgUnitOfPublication 4b6428c6-1fda-4a40-b375-456d49d2fb80
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