New Approach to Electron Microscopy Imaging of Gel Nanocomposites in situ Londono-Calderon, Alejandra Nayak, Srikanth Mosher, Curtis Mallapragada, Surya Prozorov, Tanya Mallapragada, Surya
dc.contributor.department Ames Laboratory
dc.contributor.department Neuroscience
dc.contributor.department Materials Science and Engineering
dc.contributor.department Chemical and Biological Engineering 2019-03-08T20:06:47.000 2020-06-30T01:09:57Z 2020-06-30T01:09:57Z Tue Jan 01 00:00:00 UTC 2019 2021-02-20 2019-01-01
dc.description.abstract <p>Characterization of Au-nanocomposites is routinely done with scattering techniques where the structure and ordering of nanoparticles can be analyzed. Imaging of Poloxamer gel-based Au-nanocomposites is usually limited to cryo-TEM imaging of cryo-microtomed thin sections of the specimen. While this approach is applicable for imaging of the individual nanoparticles and gauging their size distribution, it requires altering the state of the specimen and is prone to artifacts associated with preparation protocols. Use of Scanning Transmission Electron Microscopy (S/TEM) with fluid cell in situ provides an opportunity to analysis of these complex materials in their hydrated state with nanometer resolution, yet dispensing dense gel-based samples onto electron-transparent substrates remains challenging. We show that Poloxamer gel-based Au nanocomposites exhibiting thermoreversible behavior can be imaged in a fully hydrated state using a commercially available fluid cell holder, and we describe a specimen preparation method for depositing femtoliter amounts of gel-based nanocomposites directly onto the 50 nm-thick SiN window membranes. Ultimately, fluid cell S/TEM in situ imaging approach offers a pathway to visualization of individual nanoparticles within a thick gel media while maintaining the hydrated state of the carrier polymeric matrix.</p>
dc.description.comments <p>This is a manuscript of an article published as Londono-Calderon, Alejandra, Srikanth Nayak, Curtis L. Mosher, Surya K. Mallapragada, and Tanya Prozorov. "New Approach to Electron Microscopy Imaging of Gel Nanocomposites <em>in situ</em>." <em>Micron</em> (2019). DOI: <a href="" target="_blank">10.1016/j.micron.2019.02.010</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 1362
dc.identifier.contextkey 13935944
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cbe_pubs/361
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 23:47:29 UTC 2022
dc.source.uri 10.1016/j.micron.2019.02.010
dc.subject.disciplines Biology and Biomimetic Materials
dc.subject.disciplines Chemical Engineering
dc.subject.disciplines Nanoscience and Nanotechnology
dc.subject.keywords fluid cell S/TEM
dc.subject.keywords gel-based
dc.subject.keywords nanocomposite
dc.subject.keywords nanoparticle imaging
dc.subject.keywords signal-to-noise ratio
dc.title New Approach to Electron Microscopy Imaging of Gel Nanocomposites in situ
dc.type article
dc.type.genre article
dspace.entity.type Publication
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