Structural and functional studies on SNAREs-mediated membrane fusion

dc.contributor.advisor Yeon-Kyun Shin
dc.contributor.author Chen, Yong
dc.contributor.department Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology (LAS)
dc.date 2018-08-24T23:26:58.000
dc.date.accessioned 2020-07-02T05:33:44Z
dc.date.available 2020-07-02T05:33:44Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2006
dc.date.issued 2006-01-01
dc.description.abstract <p>Membrane fusion is an essential biological process for vesicle trafficking in eukaryotic cells. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) are a family of proteins that have been recognized as key components of protein complexes that drive membrane fusion. The SNARE proteins localized in opposite membranes form a SNARE complex, which bridges the two membranes into close proximity and promotes fusion. Despite considerable sequence divergence among SNARE proteins, this fusion machinery is conserved structurally, mechanistically and evolutionally from yeast to human;Site-directed spin labeling (SDSL) and electron paramagnetic resonance (EPR) are well established techniques to study the structures of membrane proteins. Fluorescent labeled lipids allow us to test the function of SNARE proteins by lipid mixing assay. With these techniques, we are able to study the structures and functions of SNAREs in vitro. In this dissertation, three sets of SNARE proteins coming from yeast, plant and mammalian sources have been investigated. The information gathered from these systems will help to understand the molecular mechanism of SNARE-induced membrane fusion.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/3085/
dc.identifier.articleid 4084
dc.identifier.contextkey 6160674
dc.identifier.doi https://doi.org/10.31274/rtd-180813-215
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/3085
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/75569
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/3085/3243567.PDF|||Fri Jan 14 23:29:35 UTC 2022
dc.subject.disciplines Biophysics
dc.subject.keywords Biochemistry
dc.subject.keywords biophysics
dc.subject.keywords and molecular biology
dc.subject.keywords Biophysics
dc.title Structural and functional studies on SNAREs-mediated membrane fusion
dc.type dissertation
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication faf0a6cb-16ca-421c-8f48-9fbbd7bc3747
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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