Effect of Macrolide Usage on Emergence of Erythromycin-Resistant Campylobacter Isolates in Chickens

dc.contributor.author Lin, Jun
dc.contributor.author Yan, Meiguan
dc.contributor.author Zhang, Qijing
dc.contributor.author Sahin, Orhan
dc.contributor.author Pereira, Sonia
dc.contributor.author Chang, Yun-Juan
dc.contributor.author Zhang, Qijing
dc.contributor.author Zhang, Qijing
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-18T23:27:15.000
dc.date.accessioned 2020-07-07T05:14:44Z
dc.date.available 2020-07-07T05:14:44Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2007
dc.date.issued 2007-05-01
dc.description.abstract <p>In this work we conducted both in vitro and in vivo experiments to examine the development and mechanisms of erythromycin (Ery) resistance in <em>Campylobacter jejuni</em> and <em>Campylobacter coli</em>. In vitro plating revealed that both <em>Campylobacter</em> species had similar but low spontaneous mutation frequencies (3 × 10<sup>−9</sup> to −10) for Ery resistance. Chickens infected with <em>C. jejuni</em> or <em>C. coli</em> were subjected to single or multiple treatments with medicated water containing tylosin (0.53 g/liter), which transiently reduced the level of <em>Campylobacter</em> colonization but did not select for Ery-resistant (Ery<sup>r</sup>) mutants in the treated birds. However, when tylosin was given to the chickens in feed at a growth-promoting dose (0.05 g/kg feed), Ery<sup>r</sup> mutants emerged in the birds after prolonged exposure to the antibiotic. The vast majority of the in vitro- and in vivo-selected <em>Campylobacter</em> mutants with Ery MICs of 8 to 256 μg/ml lacked the known resistance-associated mutations in the 23S rRNA gene, while the highly resistant mutants (Ery MIC > 512 μg/ml) had the A2074G mutation in the 23S rRNA gene. Inactivation of CmeABC, a multidrug efflux pump, dramatically reduced the Ery MIC in all of the examined mutants regardless of the presence of the A2074G mutation. Together, these results reveal distinct features associated with Ery resistance development in <em>Campylobacter</em>, demonstrate the significant role of CmeABC in Ery resistance, and suggest that long-term use of a macrolide as a growth promoter selects for the emergence of Ery<sup>r</sup> <em>Campylobacter</em> in animal reservoirs.</p>
dc.description.comments <p>This article is published as Lin, Jun, Meiguan Yan, Orhan Sahin, Sonia Pereira, Yun-Juan Chang, and Qijing Zhang. "Effect of macrolide usage on emergence of erythromycin-resistant Campylobacter isolates in chickens." Antimicrobial agents and chemotherapy 51, no. 5 (2007): 1678-1686. doi:<a href="http://dx.doi.org/10.1128/AAC.01411-06%20" target="_blank" title=" Effect of Macrolide Usage on Emergence of Erythromycin-Resistant Campylobacter Isolates in Chickens">10.1128/AAC.01411-06</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/168/
dc.identifier.articleid 1168
dc.identifier.contextkey 10836938
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/168
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92274
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/168/2007_Zhang_EffectMacrolide.pdf|||Fri Jan 14 21:06:13 UTC 2022
dc.source.uri 10.1128/AAC.01411-06
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Molecular Genetics
dc.subject.disciplines Poultry or Avian Science
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Effect of Macrolide Usage on Emergence of Erythromycin-Resistant Campylobacter Isolates in Chickens
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1c6a5dfc-c604-457f-85be-122910db782e
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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