Expression of select immune genes (surfactant proteins A and D, sheep beta defensin 1, and toll-like receptor 4) by respiratory epithelia is developmentally regulated in the preterm neonatal lamb

dc.contributor.author Gallup, Jack
dc.contributor.author Meyerholz, David
dc.contributor.author Kawashima, Kenji
dc.contributor.author Gallup, Jack
dc.contributor.author Ackermann, Mark
dc.contributor.author Grubor, Branka
dc.contributor.author Ackermann, Mark
dc.contributor.department Veterinary Pathology
dc.date 2018-02-13T06:22:32.000
dc.date.accessioned 2020-07-07T05:15:50Z
dc.date.available 2020-07-07T05:15:50Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2006
dc.date.embargo 2013-02-26
dc.date.issued 2006-01-01
dc.description.abstract <p>Preterm infants experience enhanced susceptibility and severity to respiratory syncytial virus (RSV) infection. Terminal airway epithelium is an important site of RSV infection and the extent of local innate immune gene expression is poorly understood. In this study, expression of surfactant proteins A and D (SP-AD), sheep beta defensin 1 (SBD1), and toll-like receptor 4 (TLR4) mRNA were determined in whole lung homogenates from lambs. SP-AD and TLR4 mRNA expression increased (<em>p</em><0.05) from late gestation to term birth. In addition, gene expression of LCM-retrieved type II pneumocytes (CD208+), adjacent epithelium (CD208−) and bronchial epithelium demonstrated that bronchiole-alveolar junction epithelium (combined CD208+/−) had significant (<em>p</em><0.05) developmental increases in SP-AD, SBD1 and TLR4 mRNA, whereas CD208+ cells had statistically significant increases only with SP-A mRNA. Using immunofluorescence, SP-AD antigen distribution and intensity were also greater with developmental age. These studies show reduced SBD1, SP-AD, and TLR4 expression in the preterm lung and this may underlie enhanced RSV susceptibility.</p>
dc.description.comments <p>This is a post-print of an article from <em>Developmental and Comparative Immunology </em>30, no. 11 (2006): 1060–1069, doi:<a href="http://dx.doi.org/10.1016%2Fj.dci.2006.01.001" target="_blank">10.1016/j.dci.2006.01.001</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/30/
dc.identifier.articleid 1030
dc.identifier.contextkey 3789503
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/30
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92454
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/30/2006_Meyerholz_ExpressionSelectImmune.pdf|||Fri Jan 14 23:26:39 UTC 2022
dc.source.uri 10.1016/j.dci.2006.01.001
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords Antimicrobial peptide
dc.subject.keywords Beta-defensin
dc.subject.keywords Lung
dc.subject.keywords Preterm infant
dc.subject.keywords Sheep
dc.subject.keywords Surfactant protein
dc.subject.keywords Toll-like receptor
dc.title Expression of select immune genes (surfactant proteins A and D, sheep beta defensin 1, and toll-like receptor 4) by respiratory epithelia is developmentally regulated in the preterm neonatal lamb
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isAuthorOfPublication 86c1ed73-b60d-48ce-8f35-449bc320a693
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
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