Quantitative trait loci identified for blood chemistry components of an advanced intercross line of chickens under heat stress

dc.contributor.author Rothschild, Max
dc.contributor.author Ashwell, Christopher
dc.contributor.author Persia, Michael
dc.contributor.author Lamont, Susan
dc.contributor.author Schmidt, Carl
dc.contributor.author Lamont, Susan
dc.contributor.department Animal Science
dc.date 2018-03-02T16:35:50.000
dc.date.accessioned 2020-06-29T23:40:12Z
dc.date.available 2020-06-29T23:40:12Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-01-01
dc.description.abstract <p>Background: Heat stress in poultry results in considerable economic losses and is a concern for both animal health and welfare. Physiological changes occur during periods of heat stress, including changes in blood chemistry components. A highly advanced intercross line, created from a broiler (heat susceptible) by Fayoumi (heat resistant) cross, was exposed to daily heat cycles for seven days starting at 22 days of age. Blood components measured pre-heat treatment and on the seventh day of heat treatment included pH, pCO2, pO2, base excess, HCO3, TCO2, K, Na, ionized Ca, hematocrit, hemoglobin, sO2, and glucose. A genome-wide association study (GWAS) for these traits and their calculated changes was conducted to identify quantitative trait loci (QTL) using a 600 K SNP panel.</p> <p>Results: There were significant increases in pH, base excess, HCO3, TCO2, ionized Ca, hematocrit, hemoglobin, and sO2, and significant decreases in pCO2 and glucose after 7 days of heat treatment. Heritabilities ranged from 0.01-0.21 for pre-heat measurements, 0.01-0.23 for measurements taken during heat, and 0.00-0.10 for the calculated change due to heat treatment. All blood components were highly correlated within measurement days, but not correlated between measurement days. The GWAS revealed 61 QTL for all traits, located on GGA (Gallus gallus chromosome) 1, 3, 6, 9, 10, 12–14, 17, 18, 21–28, and Z. A functional analysis of the genes in these QTL regions identified the Angiopoietin pathway as significant. The QTL that co-localized for three or more traits were on GGA10, 22, 26, 28, and Z and revealed candidate genes for birds’ response to heat stress.</p> <p>Conclusions: The results of this study contribute to our knowledge of levels and heritabilities of several blood components of chickens under thermoneutral and heat stress conditions. Most components responded to heat treatment. Mapped QTL may serve as markers for genomic selection to enhance heat tolerance in poultry. The Angiopoietin pathway is likely involved in the response to heat stress in chickens. Several candidate genes were identified, giving additional insight into potential mechanisms of physiologic response to high ambient temperatures.</p>
dc.description.comments <p>This article is published as Van Goor, Angelica, Christopher M. Ashwell, Michael E. Persia, Max F. Rothschild, Carl J. Schmidt, and Susan J. Lamont. "Quantitative trait loci identified for blood chemistry components of an advanced intercross line of chickens under heat stress." <em>BMC genomics</em> 17 (2016): 287. doi: <a href="https://doi.org/10.1186/s12864-016-2601-x" target="_blank">10.1186/s12864-016-2601-x</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/360/
dc.identifier.articleid 1357
dc.identifier.contextkey 11686493
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/360
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9781
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/360/2016_Rothschild_QuantitativeTrait.pdf|||Fri Jan 14 23:47:18 UTC 2022
dc.source.uri 10.1186/s12864-016-2601-x
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Poultry or Avian Science
dc.title Quantitative trait loci identified for blood chemistry components of an advanced intercross line of chickens under heat stress
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication a5915699-0c8a-411e-a601-1564d7090ab9
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
File
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
2016_Rothschild_QuantitativeTrait.pdf
Size:
2.35 MB
Format:
Adobe Portable Document Format
Description:
Collections