Placenta‐on‐a‐Chip: In Vitro Study of Caffeine Transport across Placental Barrier Using Liquid Chromatography Mass Spectrometry

dc.contributor.author Pemathilaka, Rajeendra
dc.contributor.author Caplin, Jeremy
dc.contributor.author Aykar, Saurabh
dc.contributor.author Montazami, Reza
dc.contributor.author Hashemi, Nicole
dc.contributor.author Hashemi, Nicole
dc.contributor.department Ames National Laboratory
dc.contributor.department Biomedical Sciences
dc.contributor.department Mechanical Engineering
dc.contributor.department Bioeconomy Institute (BEI)
dc.date 2019-03-20T01:18:50.000
dc.date.accessioned 2020-06-30T06:04:57Z
dc.date.available 2020-06-30T06:04:57Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.issued 2019-03-01
dc.description.abstract <p>Due to the particular structure and functionality of the placenta, most current human placenta drug testing methods are limited to animal models, conventional cell testing, and cohort/controlled testing. Previous studies have produced inconsistent results due to physiological differences between humans and animals and limited availability of human and/or animal models for controlled testing. To overcome these challenges, a placenta‐on‐a‐chip system is developed for studying the exchange of substances to and from the placenta. Caffeine transport across the placental barrier is studied because caffeine is a xenobiotic widely consumed on a daily basis. Since a fetus does not carry the enzymes that inactivate caffeine, when it crosses a placental barrier, high caffeine intake may harm the fetus, so it is important to quantify the rate of caffeine transport across the placenta. In this study, a caffeine concentration of 0.25 mg mL−1 is introduced into the maternal channel, and the resulting changes are observed over a span of 7.5 h. A steady caffeine concentration of 0.1513 mg mL−1 is reached on the maternal side after 6.5 h, and a 0.0033 mg mL−1 concentration on the fetal side is achieved after 5 h.</p>
dc.description.comments <p>This is the published version of the following article: Pemathilaka, Rajeendra L., Jeremy D. Caplin, Saurabh S. Aykar, Reza Montazami, and Nicole N. Hashemi. "Placenta‐on‐a‐Chip: In Vitro Study of Caffeine Transport across Placental Barrier Using Liquid Chromatography Mass Spectrometry." <em>Global Challenges</em> (2019): 1800112. DOI: <a href="http://dx.doi.org/10.1002/gch2.201800112" target="_blank">10.1002/gch2.201800112</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/me_pubs/336/
dc.identifier.articleid 1338
dc.identifier.contextkey 13995817
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath me_pubs/336
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/55205
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/me_pubs/336/2019_HashemiNicole_PlacentaChip.pdf|||Fri Jan 14 23:39:35 UTC 2022
dc.source.uri 10.1002/gch2.201800112
dc.subject.disciplines Biomechanical Engineering
dc.subject.disciplines Biomechanics and Biotransport
dc.subject.keywords caffeine transport
dc.subject.keywords in vitro
dc.subject.keywords microfluidics
dc.subject.keywords placenta‐on‐a‐chip
dc.title Placenta‐on‐a‐Chip: In Vitro Study of Caffeine Transport across Placental Barrier Using Liquid Chromatography Mass Spectrometry
dc.type article
dc.type.genre article
dspace.entity.type Publication
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