1.55 Å-resolution structure of ent-copalyl diphosphate synthase and exploration of general acid function by site-directed mutagenesis

dc.contributor.author Köksal, Mustafa
dc.contributor.author Potter, Kevin
dc.contributor.author Peters, Reuben
dc.contributor.author Christianson, David
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.date 2020-04-02T21:00:12.000
dc.date.accessioned 2020-06-29T23:47:35Z
dc.date.available 2020-06-29T23:47:35Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.issued 2014-01-01
dc.description.abstract <p>Background—The diterpene cyclase ent-copalyl diphosphate synthase (CPS) catalyzes the first committed step in the biosynthesis of gibberellins. The previously reported 2.25 Å resolution crystal structure of CPS complexed with (S)-15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate (1) established the αβγ domain architecture, but ambiguities regarding substrate analog binding remained.</p> <p>Method—Use of crystallization additives yielded CPS crystals diffracting to 1.55 Å resolution. Additionally, active site residues that hydrogen bond with D379, either directly or through hydrogen bonded water molecules, were probed by mutagenesis.</p> <p>Results—This work clarifies structure-function relationships that were ambiguous in the lower resolution structure. Well-defined positions for the diphosphate group and tertiary ammonium cation of 1 as well as extensive solvent structure, are observed.</p> <p>Conclusions—Two channels involving hydrogen bonded solvent and protein residues lead to the active site, forming hydrogen bonded "proton wires" that link general acid D379 with bulk solvent. These proton wires may facilitate proton transfer with the general acid during catalysis. Activity measurements made with mutant enzymes indicate that N425, which donates a hydrogen bond directly to D379, and T421, which hydrogen bonds with D379 through an intervening solvent molecule, help orient D379 for catalysis. Residues involved in hydrogen bonds with the proton wire, R340 and D503, are also important. Finally, conserved residue E211, which is located near the diphosphate group of 1 is proposed to be a ligand to Mg2+ required for optimal catalytic activity.</p> <p>General Significance—This work establishes structure-function relationships for class II terpenoid cyclases.</p>
dc.description.comments <p>This is the author’s version of a work that was accepted for publication in Biochimica et Biophysica Acta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochimica et Biophysica Acta, VOL 1840, ISSUE 1, 2014, DOI: <a href="https://doi.org/10.1016/j.bbagen.2013.09.004" id="x-x-x-ddDoi">10.1016/j.bbagen.2013.09.004</a>.</p>
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dc.identifier archive/lib.dr.iastate.edu/bbmb_ag_pubs/87/
dc.identifier.articleid 1103
dc.identifier.contextkey 10302703
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath bbmb_ag_pubs/87
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/10822
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/87/2014_Peters_ResolutionStructure.pdf|||Sat Jan 15 02:15:38 UTC 2022
dc.source.uri 10.1016/j.bbagen.2013.09.004
dc.subject.disciplines Biochemistry
dc.subject.disciplines Molecular Biology
dc.subject.disciplines Structural Biology
dc.subject.keywords protein crystallography
dc.subject.keywords terpene cyclase
dc.subject.keywords enzyme mechanism
dc.subject.keywords gibberellin biosynthesis
dc.title 1.55 Å-resolution structure of ent-copalyl diphosphate synthase and exploration of general acid function by site-directed mutagenesis
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 498a24ec-81d7-4bee-b145-323d38e7a392
relation.isOrgUnitOfPublication c70f85ae-e0cd-4dce-96b5-4388aac08b3f
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