Anti-Campylobacter Activities and Resistance Mechanisms of Natural Phenolic Compounds in Campylobacter

dc.contributor.author Klančnik, Anja
dc.contributor.author Možina, Sonja
dc.contributor.author Zhang, Qijing
dc.contributor.author Zhang, Qijing
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-17T11:12:44.000
dc.date.accessioned 2020-07-07T05:14:29Z
dc.date.available 2020-07-07T05:14:29Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2012
dc.date.issued 2012-12-17
dc.description.abstract <p><strong>Background</strong></p> <p>Campylobacter is a major foodborne pathogen and alternative antimicrobials are needed to prevent or decrease Campylobacter contamination in foods or food producing animals. The objectives of this study are to define the anti-Campylobacter activities of natural phenolic compounds of plant origin and to determine the roles of bacterial drug efflux systems in the resistance to these natural phenolics in Campylobacter jejuni.</p> <p><strong>Methodology/Principal Findings</strong></p> <p>Anti-Campylobacter activities were evaluated by an MIC assay using microdilution coupled with ATP measurement. Mutants of the cmeB and cmeF efflux genes and the cmeR transcriptional repressor gene were compared with the wild-type strain for their susceptibilities to phenolics in the absence and presence of efflux-pump inhibitors (EPIs). The phenolic compounds produced significant, but variable activities against both antibiotic-susceptible and antibiotic resistant Campylobacter. The highest anti-Campylobacter activity was seen with carnosic and rosmarinic acids in their pure forms or in enriched plant extracts. Inactivation of cmeB rendered C. jejuni significantly more susceptible to the phenolic compounds, while mutation of cmeF or cmeR only produced a moderate effect on the MICs. Consistent with the results from the efflux pump mutants, EPIs, especially phenylalanine-arginine β-naphthylamide and NMP, significantly reduced the MICs of the tested phenolic compounds. Further reduction of MICs by the EPIs was also observed in the cmeB and cmeF mutants, suggesting that other efflux systems are also involved in Campylobacter resistance to phenolic compounds.</p> <p><strong>Conclusion/Significance</strong></p> <p>Natural phenolic compounds of plant origin have good anti-Campylobacter activities and can be further developed for potential use in controlling Campylobacter. The drug efflux systems in Campylobacter contribute significantly to its resistance to the phenolics and EPIs potentiate the anti-Campylobacter activities of plant phenolic compounds.</p>
dc.description.comments <p>This article is from PLoS ONE 7(12): e51800. doi:<a href="http://dx.doi.org/10.1371/journal.pone.005180" target="_blank">10.1371/journal.pone.0051800</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/132/
dc.identifier.articleid 1127
dc.identifier.contextkey 8049163
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/132
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92235
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/132/2012_Klancnik_AntiCampylobacterActivities.pdf|||Fri Jan 14 19:46:56 UTC 2022
dc.source.uri 10.1371/journal.pone.0051800
dc.subject.disciplines Animal Diseases
dc.subject.disciplines Bacteriology
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.disciplines Veterinary Toxicology and Pharmacology
dc.subject.keywords Phenols
dc.subject.keywords Campylobacter
dc.subject.keywords Antimicrobials
dc.subject.keywords Antimicrobial resistance
dc.subject.keywords Antibiotics
dc.subject.keywords Polymerase chain reaction
dc.subject.keywords Antibiotic resistance
dc.subject.keywords Mutant strains
dc.title Anti-Campylobacter Activities and Resistance Mechanisms of Natural Phenolic Compounds in Campylobacter
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1c6a5dfc-c604-457f-85be-122910db782e
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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