Gametophytic Self-Incompatibility Is Operative in Miscanthus sinensis (Poaceae) and Is Affected by Pistil Age

Date
2017-01-01
Authors
Jiang, Jianziong
McCormick, Sheila
Juvik, Jack
Lubberstedt, Thomas
Fei, Shui-zhang
Lubberstedt, Thomas
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Agronomy
Organizational Unit
Horticulture
Organizational Unit
Journal Issue
Series
Department
AgronomyHorticulture
Abstract

Miscanthus sinensis Anderss. (Poaceae) has desirable traits for a dedicated biomass crop. An important breeding goal for M. sinensis is to develop F1 hybrid cultivars. A clear understanding of its reproductive mode will help to identify effective breeding strategies toward that goal. We performed >1000 semi-in-vivo reciprocal crosses and self-pollinations to determine pollen–pistil compatibility responses. Self-pollination showed a self-incompatibility (SI) response typical for grasses, indicating that SI is operative in M. sinensis. The majority of self-pollen produced short tubes that terminated at the stigmatic surface of mature pistils, but some self-pollen tubes entered into the transmitting tract. The developmental stage of pistils affects pollen–pistil interactions, as younger pistils allowed significantly more self-pollen to enter the transmitting tract, with some even reaching the ovule. Cross pollinations among progeny of reciprocal crosses between the cultivar Gross Fontaine and the cultivar Undine showed four classes of compatibility responses with 0, 50, 75, and 100% compatible pollen and exhibited differences in reciprocal compatibility for certain crosses. Taken together, our results showed that SI in M. sinensis is gametophytic and is likely controlled by a multiallelic, two-locus S and Z system, similar to those reported for other grasses. The findings from this study will facilitate the identification and isolation of genes related to SI and ultimately F1 hybrid production in M. sinensis.

Comments

This article is published as Jiang, Jianxiong, Yuefeng Guan, Sheila McCormick, Jack Juvik, Thomas Lubberstedt, and Shui-zhang Fei. "Gametophytic Self-Incompatibility Is Operative in (Poaceae) and Is Affected by Pistil Age." Crop Science (2017). 10.2135/cropsci2016.11.0932. Posted with permission.

Description
Keywords
Citation
DOI
Collections