RNAseq Reveals Complex Response of Campylobacter jejuni to Ovine Bile and In vivo Gallbladder Environment

dc.contributor.author Plummer, Paul
dc.contributor.author Kreuder, Amanda
dc.contributor.author Schleining, Jennifer
dc.contributor.author Yaeger, Michael
dc.contributor.author Zhang, Qijing
dc.contributor.author Plummer, Paul
dc.contributor.author Zhang, Qijing
dc.contributor.department Veterinary Pathology
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2018-02-19T05:26:09.000
dc.date.accessioned 2020-07-07T05:13:45Z
dc.date.available 2020-07-07T05:13:45Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2017
dc.date.issued 2017-05-01
dc.description.abstract <p>Colonization of the gallbladder by enteric pathogens such as <em>Salmonella typhi, Listeria monocytogenes</em>, and <em>Campylobacter jejuni</em> is thought to play a key role in transmission and persistence of these important zoonotic agents; however, little is known about the molecular mechanisms that allow for bacterial survival within this harsh environment. Recently, a highly virulent <em>C. jejuni</em> sheep abortion (SA) clone represented by the clinical isolate IA3902 has emerged as the dominant cause for sheep abortion in the United States. Previous studies have indicated that the <em>C. jejuni</em> clone SA can frequently be isolated from the gallbladders of otherwise healthy sheep, suggesting that the gallbladder may serve as an important reservoir for infection. To begin to understand the molecular mechanisms associated with survival in the host gallbladder, <em>C. jejuni</em> IA3902 was exposed for up to 24 h to both the natural ovine host <em>in vivo</em> gallbladder environment, as well as ovine bile <em>in vitro</em>. Following exposure, total RNA was isolated from the bile and high throughput deep sequencing of strand specific rRNA-depleted total RNA was used to characterize the transcriptome of IA3902 under these conditions. Our results demonstrated for the first time the complete transcriptome of <em>C. jejuni</em> IA3902 during exposure to an important host environment, the sheep gallbladder. Exposure to the host environment as compared to <em>in vitro</em> bile alone provided a more robust picture of the complexity of gene regulation required for survival in the host gallbladder. A subset of genes including a large number of protein coding genes as well as seven previously identified non-coding RNAs were confirmed to be differentially expressed within our data, suggesting that they may play a key role in adaptation upon exposure to these conditions. This research provides valuable insights into the molecular mechanisms that may be utilized by <em>C. jejuni</em> IA3902 to colonize and survive within the inhospitable gallbladder environment.</p>
dc.description.comments <p>This article is published as Kreuder, Amanda J., Jennifer A. Schleining, Michael Yaeger, Qijing Zhang, and Paul J. Plummer. "RNAseq reveals complex response of Campylobacter jejuni to ovine bile and in vivo gallbladder environment." Frontiers in Microbiology 8 (2017): 940. doi: <a href="https://doi.org/10.3389/fmicb.2017.00940">10.3389/fmicb.2017.00940</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/91/
dc.identifier.articleid 1090
dc.identifier.contextkey 11171622
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/91
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92122
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/91/2017_Plummer_RevealsComplex.pdf|||Sat Jan 15 02:28:31 UTC 2022
dc.source.uri 10.3389/fmicb.2017.00940
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords Campylobacter
dc.subject.keywords RNAseq
dc.subject.keywords transcriptome
dc.subject.keywords in vivo model
dc.subject.keywords gallbladder
dc.subject.keywords bile
dc.title RNAseq Reveals Complex Response of Campylobacter jejuni to Ovine Bile and In vivo Gallbladder Environment
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication f16d5130-7036-437d-892d-b4a4072903e7
relation.isAuthorOfPublication 1c6a5dfc-c604-457f-85be-122910db782e
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
File
Original bundle
Now showing 1 - 1 of 1
Name:
2017_Plummer_RevealsComplex.pdf
Size:
1.53 MB
Format:
Adobe Portable Document Format
Description:
Collections