Characterizing the Metabolic Fingerprint and Anti-inflammatory Activity of Hypericum gentianoides

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2008-01-01
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Hillwig, Matthew
Hammer, Kimberly
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Birt, Diane
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Wurtele, Eve
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In this paper we characterize the metabolic fingerprint and first reported anti-inflammatory activity of Hypericum gentianoides. H. gentianoides has a history of medical use by Native Americans, but it has been studied very little for biological activity. High-performance liquid chromatography (HPLC) and liquid chromatography−electrospray ionization−mass spectrometry (LC-ESI-MS) analyses of a methanol extract show that H. gentianoides contains a family of over nine related compounds that have retention times, mass spectra, and a distinctive UV absorption spectra characteristic of certain acyl-phloroglucinols. These metabolites are abundant relative to other secondary products present in H. gentianoides, accounting for approximately 0.2 g per gram of dry plant tissue. H. gentianoides methanol extracts and a specific semipreparative HPLC fraction from these extracts containing the putative acyl-phloroglucinols reduce prostaglandin E2 synthesis in mammalian macrophages.

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Posted with permission from Journal of Agricultural and Food Chemistry 56, no. 12 (2008): 4359–4366, doi:10.1021/jf800411v. Copyright 2008 American Chemical Society.

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Tue Jan 01 00:00:00 UTC 2008
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