The production and characterization of anti-idiotypic antibodies to syngeneic monoclonal and xenogeneic polyclonal antibodies to soybean mosaic virus

dc.contributor.advisor Donald P. Durand
dc.contributor.author Mernaugh, Raymond
dc.contributor.department Microbiology
dc.date 2018-08-16T19:54:57.000
dc.date.accessioned 2020-07-02T06:12:27Z
dc.date.available 2020-07-02T06:12:27Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 1987
dc.date.issued 1987
dc.description.abstract <p>Soybean mosaic virus (SMV)-mimicking anti-idiotypic antibodies (Abs) were developed for use as positive controls in enzyme linked immunosorbent assays (ELISAs) and as probes for identification of putative SMV receptors in plants;Anti-idiotypic monoclonal antibodies (McAbs) were generated against either the anti-SMV McAb S1 or against rabbit anti-SMV polyclonal antibodies (SMV Abs);A syngeneic IgM anti-S1 idiotope hybridoma designated 1a produced McAbs which recognized intraspecies, cross-reactive idiotopes (CRIs) on the IgG2a kappa light chain McAbs S1 and NDV4F11, an anti-Newcastle's disease virus McAb. It also recognized an interspecies CRI on SMV Abs. The 1a McAb did not react with all IgG2a kappa light chain McAbs nor did it react with rabbit polyclonal Abs to two other plant viruses;S1 or SMV competed with SMV Abs for bound 1a in ELISAs. This indicated that S1 and SMV Abs have nearly identical CRIs and that 1a recognized an idiotope near the paratope of SMV Abs. Since 1a recognized both SMV and S1, it exhibited features characteristic of an epibody;Since 1a, like bound SMV, captured SMV Abs in ELISAs, it was possible to use an anti-idiotope Ab such as 1a as a positive control in such assays;Four anti-SMV Ab hybridomas produced Abs which in ELISAs, reacted to a greater extent with SMV Abs than with control Abs or SMV Abs in the presence of SMV. In preliminary experiments, these hybridoma Abs appeared to mimic SMV and to inhibit SMV local lesion formation on detached Phaseolus vulgaris L. C.V. Top crop leaves. Whether this inhibition is specific due to hybridoma Abs mimicking SMV or is a non-specific phenomenon has yet to be determined.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/9280/
dc.identifier.articleid 10279
dc.identifier.contextkey 6355916
dc.identifier.doi https://doi.org/10.31274/rtd-180813-9092
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/9280
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/82362
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/9280/r_8805115.pdf|||Sat Jan 15 02:30:45 UTC 2022
dc.subject.disciplines Microbiology
dc.subject.keywords Microbiology
dc.title The production and characterization of anti-idiotypic antibodies to syngeneic monoclonal and xenogeneic polyclonal antibodies to soybean mosaic virus
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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