EGF-induced Contraction Regulates Paxillin Phosphorylation to Temporally Separate Traction Generation from De-adhesion

dc.contributor.author Schneider, Ian
dc.contributor.author Hays, Cristen
dc.contributor.author Waterman, Clare
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Chemical and Biological Engineering
dc.date 2020-07-11T03:50:43.000
dc.date.accessioned 2021-02-24T22:09:11Z
dc.date.available 2021-02-24T22:09:11Z
dc.date.issued 2009-07-01
dc.description.abstract <p>Directed cell migration is mediated by cycles of protrusion, adhesion, traction generation on the extracellular matrix and retraction. However, how the events after protrusion are timed, and what dictates their temporal order is completely unknown. We used acute epidermal growth factor (EGF) stimulation of epidermal keratinocytes to initiate the cell migration cycle to study the mechanism of the timing of adhesion, traction generation, and de-adhesion. Using microscopic and biochemical assays, we surprisingly found that at ∼2 min after EGF stimulation protrusion, activation of myosin-II, traction generation, adhesion assembly, and paxillin phosphorylation occurred nearly simultaneously, followed by a 10-min delay during which paxillin became dephosphorylated before cell retraction. Inhibition of myosin-II blocked both the EGF-stimulated paxillin phosphorylation and cell retraction, and a paxillin phosphomimic blocked retraction. These results suggest that EGF-mediated activation of myosin-II acts as a mechanical signal to promote a cycle of paxillin phosphorylation/dephosphorylation that mediates a cycle of adhesion strengthening and weakening that delays cell retraction. Thus, we reveal for the first time a mechanism by which cells may temporally segregate protrusion, adhesion, and traction generation from retraction during EGF-stimulated cell migration.</p>
dc.description.comments <p>This article is published as Schneider, Ian C., Cristen K. Hays, and Clare M. Waterman. "EGF-induced Contraction Regulates Paxillin Phosphorylation to Temporally Separate Traction Generation from De-adhesion." <em>Molecular Biology of the Cell</em> 20, no. 13 (2009): 3003-3167. DOI: <a href="https://doi.org/10.1091/mbc.e09-03-0219" target="_blank">10.1091/mbc.e09-03-0219</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/cbe_pubs/436/
dc.identifier.articleid 1436
dc.identifier.contextkey 18476433
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cbe_pubs/436
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/93593
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/436/2009_SchneiderIan_EGFInduced.pdf|||Sat Jan 15 00:16:24 UTC 2022
dc.source.uri 10.1091/mbc.e09-03-0219
dc.subject.disciplines Biological Engineering
dc.subject.disciplines Molecular, Cellular, and Tissue Engineering
dc.title EGF-induced Contraction Regulates Paxillin Phosphorylation to Temporally Separate Traction Generation from De-adhesion
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
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